Genome size estimation of liver fluke Opisthorchis viverrini by real-time polymerase chain reaction based method

被引：5

作者：

Kaewkong, Worasak ^{[1
]}

Imtawil, Kanokwan ^{[1
]}

Maleewong, Wanchai ^{[2
]}

Intapan, Pewpan M. ^{[2
]}

Sri-Aroon, Pusadee ^{[3
]}

Wongkham, Sopit ^{[1
]}

Wongkham, Chaisiri ^{[1
]}

机构：

[1] Khon Kaen Univ, Fac Med, Dept Biochem, Liver Fluke & Cholangiocarcinoma Res Ctr, Khon Kaen 40002, Thailand

[2] Khon Kaen Univ, Fac Med, Dept Parasitol, Res & Diagnost Ctr Emerging Infect Dis, Khon Kaen 40002, Thailand

[3] Mahidol Univ, Fac Trop Med, Appl Malacol Ctr, Dept Social & Environm Med, Bangkok 10400, Thailand

来源：

PARASITOLOGY INTERNATIONAL | 2012年 / 61卷 / 01期

关键词：

Opisthorchis viverrini; Cholangiocarcinoma; Genome size; C value; PCR-BASED METHOD; SEQUENCE;

D O I：

10.1016/j.parint.2011.06.025

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The human liver fluke, Opisthorchis viverrini, has been categorized as a class one carcinogenic organism according to its strong association with cholangiocarcinoma, bile duct cancer which has high incidence in the northeast of Thailand. The lack of genome database of this parasite limited the studies aimed to understand the basic molecular biology of this carcinogenic liver fluke. The determination of the genome size is an initial step prior to the full genome sequencing. In this study, we applied an absolute quantitative real-time polymerase chain reaction for this aspect. Our results indicated the genome size of O. viverrini is 75.95 Mb or C value 0.083. The information of O. viverrini genome size is useful for estimation of sequence coverage and the cost of the parasite's whole genome sequencing using next-generation sequencing technologies. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

引用

页码：77 / 80

页数：4