Isolation of 60 polymorphic microsatellite loci in EST libraries of four sibling species of the phytopathogenic fungal complex Microbotryum

被引：21

作者：

Giraud, T. ^{[1
]}

Yockteng, R. ^{[1
]}

Marthey, S. ^{[2
]}

Chiapello, H. ^{[2
]}

Jonot, O. ^{[1
]}

Lopez-Villavicencio, M. ^{[1
]}

De Vienne, D. M. ^{[1
]}

Hood, M. E. ^{[3
]}

Refregier, G. ^{[1
]}

Gendrault-Jacquemard, A. ^{[2
]}

Wincker, P. ^{[4
]}

Dossat, C. ^{[4
]}

机构：

[1] Univ Paris Sud, ESE, F-91405 Orsay, France

[2] MIG INRA, F-78350 Jouy En Josas, France

[3] Amherst Coll, Dept Biol, Amherst, MA 01002 USA

[4] Genoscope CNRG CP 5706, F-91057 Evry, France

来源：

MOLECULAR ECOLOGY RESOURCES | 2008年 / 8卷 / 02期

关键词：

basidiomycete; phytopathogen; repeats; transposable elements; Ustilago violacea;

D O I：

10.1111/j.1471-8286.2007.01967.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the development of 60 microsatellite markers on four species of the fungal complex Microbotryum, causing anther smut of the Caryophyllaceae. Microsatellites were found in four expressed sequence tag (EST) libraries, built from isolates of M. lychnis-dioicae, M. violaceum sensus stricto, M. lagerheimii and M. dianthorum, collected, respectively, from the plants Silene latifolia, S. nutans, S. vulgaris and Dianthus carthusianorum. Intrapopulation polymorphism was investigated using 24 isolates, and cross-amplification was explored using 23 isolates belonging to at least 10 different Microbotryum species. This study provides numerous microsatellite markers for population genetics and mapping studies.

引用

页码：387 / 392

页数：6

共 10 条

[1]

[Anonymous], 2007, MYCOL BALCAN

[2]

Bucheli E, 1998, MOL ECOL, V7, P665

[3] Patterns of within population dispersal and mating of the fungus Microbotryum violaceum parasitising the plant Silene latifolia [J].