Site-directed mutagenesis of large plasmids

被引：5
|
作者：
Nadin-Davis, SA [1 ]
Chang, SC [1 ]
机构：
[1] Canadian Food Inspect Agcy, Inst Anim Dis Res, Nepean, ON K2H 8P9, Canada
来源：
BIOTECHNIQUES | 1998年 / 25卷 / 06期
关键词：
D O I：
10.2144/98256st04
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A protocol combining recombination PCR and long-distance PCR is demonstrated to be highly accurate and rapid for site-directed mutagenesis of large (>10 kb) plasmids. Application of this this protocol to the generation of mutant rabies virus glycoproteins expressed by the baculovirus/insect cell system illustrates the usefulness of this approach in facilitating structure-function relationships in this important eukaryotic expression system.
引用
收藏
页码：1014 / +
页数：5
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