Genetic screens using the piggyBac transposon

被引：16

作者：

Chew, Su Kit ^{[1
]}

Rad, Roland ^{[1
]}

Futreal, P. Andrew ^{[1
]}

Bradley, Allan ^{[1
]}

Liu, Pentao ^{[1
]}

机构：

[1] Wellcome Trust Sanger Inst, Cambridge CB10 1HH, England

来源：

METHODS | 2011年 / 53卷 / 04期

基金：

英国惠康基金;

关键词：

Transposon; Transposition; piggyBac; Mouse; Mutagenesis; Cancer; SLEEPING-BEAUTY; MUTAGENESIS; DISCOVERY; TOOL;

D O I：

10.1016/j.ymeth.2010.12.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Transposons are an attractive system to use in genetic screens as they are molecularly tractable and the disrupted loci that give rise to the desired phenotype are easily mapped. We consider herein the characteristics of the piggyBac transposon system in complementing existing mammalian screen strategies, including the Sleeping Beauty transposon system. We also describe the design of the piggyBac resources that we have developed for both forward and reverse genetic screens, and the protocols we use in these experiments. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：366 / 371

页数：6

共 25 条

[1] Generation of an inducible and optimized piggyBac transposon systemy [J].

Cadinanos, Juan ;

Bradley, Allan .

NUCLEIC ACIDS RESEARCH, 2007, 35 (12)

[2] The art and design of genetic screens: maize [J].

Candela, Hector ;

Hake, Sarah .

NATURE REVIEWS GENETICS, 2008, 9 (03) :192-203

[3] Cancer gene discovery in solid tumours using transposon-based somatic mutagenesis in the mouse [J].

Collier, LS ;

Carlson, CM ;

Ravimohan, S ;

Dupuy, AJ ;

Largaespada, DA .

NATURE, 2005, 436 (7048) :272-276

[4] Detecting statistically significant common insertion sites in retroviral insertional mutagenesis screens [J].

de Ridder, Jeroen ;

Uren, Anthony ;

Kool, Jaap ;

Reinders, Marcel ;

Wessels, Lodewyk .

PLOS COMPUTATIONAL BIOLOGY, 2006, 2 (12) :1530-1542

[5] Efficient transposition of the piggyBac resource (PB) transposon in mammalian cells and mice [J].

Ding, S ;

Wu, XH ;

Li, G ;

Han, M ;

Zhuang, Y ;

Xu, T .

CELL, 2005, 122 (03) :473-483

[6] Analysis of the aphthovirus 2A/2B polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal 'skip' [J].

Donnelly, MLL ;

Luke, G ;

Mehrotra, A ;

Li, XJ ;

Hughes, LE ;

Gani, D ;

Ryan, MD .

JOURNAL OF GENERAL VIROLOGY, 2001, 82 :1013-1025

[7] Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system [J].

Dupuy, AJ ;

Akagi, K ;

Largaespada, DA ;

Copeland, NG ;

Jenkins, NA .

NATURE, 2005, 436 (7048) :221-226

[8] Precise excision of TTAA-specific lepidopteran transposons piggyBac(IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera [J].

Fraser, MJ ;

Coszczon, T ;

Elick, T ;

Bauser, C .

INSECT MOLECULAR BIOLOGY, 1996, 5 (02) :141-151

[9] Characterization of Sleeping Beauty transposition and its application to genetic screening in mice [J].

Horie, K ;

Yusa, K ;

Yae, K ;

Odajima, J ;

Fischer, SEJ ;

Keng, VW ;

Hayakawa, T ;

Mizuno, S ;

Kondoh, G ;

Ijiri, T ;

Matsuda, Y ;

Plasterk, RHA ;

Takeda, J .

MOLECULAR AND CELLULAR BIOLOGY, 2003, 23 (24) :9189-9207

[10] Molecular reconstruction of Sleeping beauty, a Tc1-like transposon from fish, and its transposition in human cells [J].

Ivics, Z ;

Hackett, PB ;

Plasterk, RH ;

Izsvak, Z .

CELL, 1997, 91 (04) :501-510

← 1 2 3 →