Alginate-gelatin based core-shell capsule enhances the osteogenic potential of human osteoblast-like MG-63 cells

被引:10
作者
Ke, Cherng-Jyh [1 ]
Chiu, Kuo-Hui [2 ]
Chen, Ching-Yun [3 ]
Huang, Chiung-Hua [4 ]
Yao, Chun-Hsu [1 ,5 ,6 ,7 ]
机构
[1] China Med Univ Hosp, Biomat Translat Res Ctr, 2 Yude Rd, Taichung 40447, Taiwan
[2] China Med Univ, Dept Biol Sci & Technol, 100,Sec 1,Jingmao Rd, Taichung 406040, Taiwan
[3] Natl Cent Univ, Dept Biomed Sci & Engn, Taoyuan 32001, Taiwan
[4] Cent Taiwan Univ Sci & Technol, Dept Med Lab Sci & Biotechnol, Taichung 40601, Taiwan
[5] China Med Univ, Dept Biomed Imaging & Radiol Sci, Taichung 40202, Taiwan
[6] China Med Univ, Sch Chinese Med, Taichung 40402, Taiwan
[7] Asia Univ, Dept Biomed Informat, Taichung 41354, Taiwan
关键词
3D culture; Core-shell capsule; Alginate; Gelatin; Osteogenesis; 3D CULTURE; COLLAGEN HYDROGEL; STEM-CELLS; ENCAPSULATION; MATRIX; SCAFFOLDS; MICROGELS;
D O I
10.1016/j.matdes.2021.110109
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Three-dimensional (3D) cell culture enhanced cells-cells communication, promoting developmental processes and influencing cell performance. In this study, 3D alginate-gelatin core-shell capsules (AGC) were fabricated by using a microfluidic device. Human osteoblast-like MG-63 cells were encapsulated within the 3D model. The morphology and physicochemical properties of the AGC were characterized by using scanning electron microscopy (SEM), Flourier Transform Infrared Spectrum (FTIR), Gravimetric thermal analyzer (TGA). The mRNA levels of osteogenetic gene markers were measured by quantitative realtime RT-PCR. The result of a 9.6% weight loss after a 7-day soaking indicates that the AGC exhibited excellent stability. The encapsulated MG-63 cells formed clusters alongside the core of AGC and obtained 200% cell viability after 21-day long-term cultivation. Compared to traditional 2D cell culture, the 3D cultured MG-63 cells performed higher alkaline phosphatase (ALP), a 9.3-fold increase of calcium mineralization activity, and enhanced osteogenic gene expression of BGLAP, COL1A1, and Runx2. These observations suggested that AGC obtains the potential as an ideal 3D microenvironmental cell culture system for biomedical application. (c) 2021 Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/).
引用
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页数:11
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