Distribution and molecular composition of heterochromatin in the holocentric chromosomes of the aphid Rhopalosiphum padi (Hemiptera: Aphididae)

被引:9
作者
Monti, Valentina [1 ]
Manicardi, Gian Carlo [2 ]
Mandrioli, Mauro [1 ]
机构
[1] Univ Modena & Reggio Emilia, Dipartimento Biol, I-41125 Modena, Italy
[2] Univ Modena & Reggio Emilia, Dipartimento Sci Agr & Alimenti, I-42122 Reggio Emilia, Italy
关键词
Aphid; Holocentric chromosomes; Heterochromatin; Satellite DNA; MEGOURA-VICIAE HOMOPTERA; SATELLITE DNA FAMILY; HIGHLY-REPEATED DNA; REPETITIVE DNA; CYTOGENETIC CHARACTERIZATION; GENOMIC ORGANIZATION; X-CHROMOSOME; SEQUENCES; EVOLUTION; TRANSCRIPTION;
D O I
10.1007/s10709-010-9493-5
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
In order to study the structure of holocentric chromosomes in aphids, the localization and the composition of Rhopalosiphum padi heterochromatin and rDNA genes have been evaluated at cytogenetic and molecular level. In particular, heterochromatin resulted located on all the chromosomes both in intercalary and telomeric positions. Moreover, enzymatic digestion of R. padi genome put in evidence a DraI satellite DNA which has been isolated, cloned and sequenced. FISH experiments showed that this satellite DNA clusters in an intercalary C-positive band on the two X chromosomes.
引用
收藏
页码:1077 / 1084
页数:8
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