Vasopressin accelerates experimental ammonia-induced brain edema in rats after portacaval anastomosis

Background/Aims: Cerebral hyperemia is an important contributor to the development of brain edema in fulminant hepatic failure. Rats receiving an ammonia infusion after portacaval anastomosis (PCA) demonstrate a rise in cerebral blood flow (CBF) with brain edema at 180 min. Vasopressin (VP), a systemic vasoconstrictor which in the rat dilates cerebral vessels through V-2 receptors, was used to ascertain the effects of increasing CBF. Methods: Changes in CBF were measured with Laser Doppler flowmetry (LDF). Absolute CBF was measured with radioactive microspheres to calculate oxygen and ammonia uptake. Results: Compared to the NH3 + Vehicle group, VP + NH3 infusion accelerated the rise in CBF (117 21 vs. - 6 +/- 12 %, P < 0.01), and the development of brain edema (81.09 +/- 0.17 vs. 80.29 +/- 0.06 %, P < 0.01). Radioactive microspheres confirmed these results (254 +/- 44 vs. 106 +/- 9.5 ml/min/100 g, P < 0.01). Oxygen uptake was similar. Ammonia uptake was more than twofold higher in the VP + NH3 group. A V, antagonist negated the higher mean arterial pressure (MAP) that occurs with VP but cerebral hyperemia still occurred. A V2 antagonist resulted in similar systemic pressures, CBF and brain water compared to the VP + NH3 group. Conclusions: In this model, an increase in CBF with VP hastens the development of brain edema while increasing ammonia delivery to the brain. (C) 2003 European Association for the Study of the Liver. Published by Elsevier Science B.V. All rights reserved.