Tetrazolium reduction as an indicator of environmental stress in lichens and isolated bionts

被引：38
作者：
Backor, M
Fahselt, D
机构：
[1] Safarik Univ, Dept Bot, Inst Biol & Ecol, Kosice 04167, Slovakia
[2] Univ Western Ontario, Dept Biol, London, ON N6A 5B7, Canada
来源：
ENVIRONMENTAL AND EXPERIMENTAL BOTANY | 2005年 / 53卷 / 02期
基金：
加拿大自然科学与工程研究理事会;
关键词：
biomonitoring; formazan; lichens; mycobionts; photobionts; respiration; copper; pH; UV; temperature;
D O I：
10.1016/j.envexpbot.2004.03.007
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
A rapid and inexpensive quantitative method was developed for evaluating the physiological condition of intact lichens and cultured symbionts. Formation of the colored product triphenyl formazan (TPF), which is produced after reduction of triphenyltetrazolium chloride (TTC), was used as an indication of dehydrogenase activity. Low pH, exposure to copper, supplemental UV and temperature extremes significantly reduced the capacity of cultured lichens and bionts to produce formazan, suggesting each of these treatments may produce physiological damage. It is projected that this method may also be suitable for monitoring impact of various environmental stresses on field-collected lichens. (c) 2004 Elsevier B.V. All rights reserved.
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收藏
页码：125 / 133
页数：9
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