Application of Alkaline Phosphatase to Assess the Health of Oreochromis niloticus Exposed to Organophosphates and Synthetic Pyrethroid Pesticides In Vivo

被引：3

作者：

Amin, Muhammad ^{[1
]}

Yousuf, Masarrat ^{[1
]}

Ahmad, Naveed ^{[2
]}

Attaullah, Mohammad ^{[1
]}

Ahmad, Saeed ^{[1
]}

Zekker, Ivar ^{[3
]}

Latif, Mehreen ^{[4
]}

Buneri, Islam Dad ^{[1
]}

Khan, Ayaz Ali ^{[5
]}

Ali, Hazrat ^{[6
]}

Ikram, Muhammad ^{[7
]}

Nabi, Ghulam ^{[8
,9
]}

Azra, Mohamad Nor ^{[10
]}

Shah, Luqman Ali ^{[11
]}

Krauklis, Andrey E. ^{[12
]}

Aouissi, Hani Amir ^{[13
]}

Burlakovs, Juris ^{[14
]}

机构：

[1] Univ Karachi, Dept Zool, Karachi 75270, Pakistan

[2] Bahria Univ, Dept Maritime Sci, Karachi 75260, Pakistan

[3] Univ Tartu, Inst Chem, 14a Ravila St, EE-50414 Tartu, Estonia

[4] Bahria Univ Med & Dent Coll, Karachi 75270, Pakistan

[5] Univ Malakand, Dept Biotechnol, Malakand 18800, Pakistan

[6] Univ Malakand, Dept Chem, Malakand 18800, Pakistan

[7] Abdul Wali Khan Univ, Dept Chem, Mardan 23200, Pakistan

[8] Polish Acad Sci, Inst Nat Conservat, PL-31120 Krakow, Poland

[9] Hebei Normal Univ, Coll Life Sci, Key Lab Anim Physiol Biochem & Mol Biol Hebei Pro, Shijiazhuang 050024, Hebei, Peoples R China

[10] Univ Malaysia, Inst Marine Biotechnol, Terengganu 21030, Malaysia

[11] Univ Peshawar, Natl Ctr Excellence Phys Chem, Peshawar, Pakistan

[12] Univ Latvia, Inst Mech Mat, Jelgavas St 3, LV-1004 Riga, Latvia

[13] Sci & Tech Res Ctr Arid Reg CRSTRA, Biskra 07000, Algeria

[14] Estonian Univ Life Sci, Rural Bldg & Water Management, Kreutzwaldi 5, EE-50414 Tartu, Estonia

来源：

JOURNAL OF HAZARDOUS TOXIC AND RADIOACTIVE WASTE | 2022年 / 26卷 / 04期

基金：

欧盟地平线“2020”;

关键词：

Pesticides; Toxicity; Biomarker; Alkaline phosphatase; Oreochromis niloticus; FRESH-WATER CATFISH; BIOCHEMICAL PARAMETERS; LAMBDA-CYHALOTHRIN; ACUTE TOXICITY; SHORT-TERM; FISH; SERUM; DELTAMETHRIN; MICRONUCLEI; ENDOSULFAN;

D O I：

10.1061/(ASCE)HZ.2153-5515.0000714

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The most widely used pesticides for residential areas and agriculture purposes, which belong to the organophosphates and synthetic pyrethroids, were evaluated in this study for their LC0 toxicity. In an aquatic environment, these pesticides are highly toxic to nontargeted organisms. Oreochromis niloticus (Nile tilapia) was selected for the bioassay tests due to its presence in the trophic levels and high bioaccumulation of the toxicants. This study evaluated the impact of pesticides on the activity of alkaline phosphatase (ALP) enzymes in the brain, gills, and muscles of O. niloticus. The fish were exposed to the LC0 concentrations of malathion (1.425 ppm), chlorpyrifos (0.125 ppm), and lambda-cyhalothrin (0.0039 ppm) for 24 and 48 h. For biochemical assays, malathion, chlorpyrifos, and lambda-cyhalothrin measurements were carried out using spectrophotometry. ALP activity in the brain and gills was elevated and showed significant inhibition (p < 0.05) at 24- and 48-h exposure, respectively. For the muscle tissues, a statistically important (p < 0.05) inhibition was recorded for the activity of ALP after 24- and 48-h treatments. The highest (+138%) and the lowest (+28%) enhancement in ALP activity were reported in the gills and brain tissues, which were treated with chlorpyrifos and malathion, respectively. The minimum and maximum reduction in ALP levels were noted in the 48- and 24-h malathion-treated muscle tissues, respectively. The data for the muscle tissue sample showed good consistency, because inhibition in ALP activity was noted. Therefore, the assessment of ALP activity in different organs and tissues in fish for the detection of chemical hazards in the environment is recommended. These findings confirmed that pesticides should be consistently monitored in the environment, to lower the pollutant stress on nontargeted organisms, especially fish, because the pesticides have a toxic effect on the health of aquatic organisms. (C) 2022 American Society of Civil Engineers.

引用

页数：8

共 64 条

[11]

Bayne B.L., 1985, The Effects of Stress and Pollution on Marine Animals

[12] Contaminant accumulation and biomarker responses in flounder (Platichthys flesus L) and Atlantic cod (Gadus morhua L) exposed by caging to polluted sediments in Sorfjorden, Norway [J].