Optimization and production of bioplastic from bio waste using response surface methodology (RSM)

The unlimited and biodegradable properties of bioplastics such as polyhydroxyalcanoytes (PHAs) create appropriate benefits instead of synthetic plastics. The final rule of this environmental friendly and nontoxic bioplastic depends on satisfactory cost and in additional efforts toward the need for execution. In this study, we statistically optimized biodegradable waste (pomegranate peel) as a single carbon/nitrogen source to manufacture PHA through Bacillus subitilis NCDC0671 in reference to an efficient waste management practice. Various parameters, such as substrate concentration, temperature, pH, and time duration, were optimized using response surface methodology (RSM) to enhance the production of bioplastic PHA. The optimized values were found to be substrate concentration-5.9 g, temperature-35 degrees C, pH-6.9, and time duration-3 days. The importance of the model was accepted by ANOVA outputs (p <= 0.05) with an R2 value of 0.9966. The lower-cost fermentation medium was created by the present inquiry using pomegranate peel produced in significant quantities of innovative PHA. Bioplastic (PHA) materials were identified using Fourier transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) was used to examine the pomegranate peel, paste, and biofilm. Product validity is performed by conducting various tests, including the degradation test, swelling test, solubility test, molding test, biodegradability test and elongation test.