Maternal and foetal cytokine production in dams naturally and experimentally infected with Neospora caninum on gestation day 110

被引：6

作者：

Darwich, L. ^{[1
,2
]}

Li, Y. ^{[1
,2
]}

Serrano-Perez, B. ^{[3
]}

Mur-Novales, R. ^{[3
]}

Garcia-Ispierto, I. ^{[3
]}

Cabezon, O. ^{[4
]}

Lopez-Gatius, F. ^{[3
]}

Almeria, S. ^{[1
,2
]}

机构：

[1] UAB, Fac Vet, Dept Sanitat & Anat Anim, Cerdanyola Del Valles 08193, Spain

[2] UAB, IRTA, Ctr Recerca Sanitat Anim CReSA, Campus UAB, Cerdanyola Del Valles 08193, Spain

[3] Univ Lleida, Dept Prod Anim, Agrotecnio Ctr, Lleida, Spain

[4] UAB, SeFAS, Bellaterra, Spain

来源：

RESEARCH IN VETERINARY SCIENCE | 2016年 / 107卷

关键词：

Neospora caninum; Experimental and natural infection; Pregnant dams; Mid-gestation; Viable and aborted foetuses; IFN-gamma; IL-17A and IL-4 levels; HOST-PARASITE RELATIONSHIP; CD4(+) T-CELLS; IMMUNE-RESPONSES; BOVINE NEOSPOROSIS; PREGNANT CATTLE; INTERFERON-GAMMA; GENE-EXPRESSION; INTRACELLULAR MULTIPLICATION; EXPERIMENTAL CHALLENGE; DAIRY-COWS;

D O I：

10.1016/j.rvsc.2016.05.003

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

In the present study, IFN-gamma (Th1), IL-17 A (Th17) and IL-4 (Th2) concentrations in response to concanavalin (ConA) and Neospora caninum antigen (Nc-1) stimulation were determined in cultures of cells from control uninfected (n = 4), naturally N. caninum-infected (n = 3) and experimentally N. caninum-infected (n = 6) pregnant dams and their foetuses. Experimental animals were infected at 110 days of gestation and euthanized 6 weeks post-infection. In culture supernatants from the dams, significantly higher IFN-gamma and IL-4 levels were found in the experimentally-infected animals compared to the control or naturally-infected dams. However, among the experimentally-infected dams no significant differences in IFN-gamma production were observed regardless of the incidence of live or aborted/dead foetuses, though spleen cultures of dams carrying live foetuses showed the highest levels of IFN-gamma. IL-17A production was very low and occasional in the dams infected with N. caninum and did not seem to be a major regulator of IFN-gamma production in this model. Experimentally infected dams with live foetuses showed higher IL-4 levels and accordingly IFN-gamma/IL-4 ratios were significantly lower than ratios recorded for cows with aborted/dead foetuses. In the infected foetuses of these dams, only spleen cultures showed high levels of IFN-gamma and 1L-4 after Nc-1 antigen and ConA stimulation, respectively. No IL-17A was detected in the foetuses. As conclusion, although we could not clearly relate a protective immune response against N. caninum abortion only to IFN-gamma levels in cell cultures, our results highlight the important role of an inverse IFN-gamma/IL-4 balance in conferring protection against abortion induced by this parasite. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：55 / 61

页数：7

共 52 条

[31] Neutralization of maternal IL-4 modulates congenital protozoal transmission: Comparison of innate versus acquired immune responses [J].

Long, MT ;

Baszler, TV .

JOURNAL OF IMMUNOLOGY, 2000, 164 (09) :4768-4774

[32] Protection against abortion linked to gamma interferon production in pregnant dairy cows naturally infected with Neospora caninum [J].

Lopez-Gatius, F. ;

Almeria, S. ;

Donofrio, G. ;

Nogareda, C. ;

Garcia-Ispierto, I. ;

Bech-Sabat, G. ;

Santolaria, P. ;

Yaniz, J. L. ;

Pabon, M. ;

de Sousa, N. M. ;

Beckers, J. F. .

THERIOGENOLOGY, 2007, 68 (07) :1067-1073

[33] Cellular immune responses in cattle experimentally infected with Neospora caninum [J].

Lundén, A ;

Marks, J ;

Maley, SW ;

Innes, EA .

PARASITE IMMUNOLOGY, 1998, 20 (11) :519-526

[34] Characterization of the immune response in the placenta of cattle experimentally infected with Neospora caninum in early gestation [J].

Maley, S. W. ;

Buxton, D. ;

Macaldowie, C. N. ;

Anderson, I. E. ;

Wright, S. E. ;

Bartley, P. M. ;

Esteban-Redondo, I. ;

Hamilton, C. M. ;

Storset, A. K. ;

Innes, E. A. .

JOURNAL OF COMPARATIVE PATHOLOGY, 2006, 135 (2-3) :130-141

[35] Identification of Neospora antigens recognized by CD4+ T cells and immune sera from experimentally infected cattle [J].

Marks, J ;

Lundén, A ;

Harkins, D ;

Innes, E .

PARASITE IMMUNOLOGY, 1998, 20 (07) :303-309

[36] Neosporosis in naturally infected pregnant dairy cattle [J].

Mazuz, Monica L. ;

Fish, Leah ;

Reznikov, Dror ;

Wolkomirsky, Ricardo ;

Leibovitz, Benjamin ;

Savitzky, Igor ;

Golenser, Jacob ;

Shkap, Varda .

VETERINARY PARASITOLOGY, 2014, 205 (1-2) :85-91

[37] Vaccines against neosporosis: What can we learn from the past studies? [J].

Monney, Thierry ;

Hemphill, Andrew .

EXPERIMENTAL PARASITOLOGY, 2014, 140 :52-70

[38] Experimental Neospora caninum infection modifies trophoblast cell populations and plasma pregnancy-associated glycoprotein 1 and 2 dynamics in pregnant dairy heifers [J].

Mur-Novales, R. ;

Serrano-Perez, B. ;

Garcia-Ispierto, I. ;

de Sousa, N. M. ;

Beckers, J. F. ;

Almeria, S. ;

Lopez-Gatius, F. .

VETERINARY PARASITOLOGY, 2016, 216 :7-12

[39] Chronic Neospora caninum infection and repeat abortion in dairy cows:: A 3-year study [J].

Pabon, M. ;

Lopez-Gatius, F. ;

Garcia-Ispierto, I. ;

Bech-Sabat, G. ;

Nogareda, C. ;

Almeria, S. .

VETERINARY PARASITOLOGY, 2007, 147 (1-2) :40-46

[40] Two distinct populations of Bovine IL-17+ T-cells can be induced and WCl +IL-17+ γδ T-cells are effective killers of protozoan parasites [J].

Peckham, R. K. ;

Brill, R. ;

Foster, D. S. ;

Bowen, A. L. ;

Leigh, J. A. ;

Coffey, T. J. ;

Flynn, R. J. .

SCIENTIFIC REPORTS, 2014, 4

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