Maternal and foetal cytokine production in dams naturally and experimentally infected with Neospora caninum on gestation day 110

被引：6
作者：
Darwich, L. [1 ,2 ]
Li, Y. [1 ,2 ]
Serrano-Perez, B. [3 ]
Mur-Novales, R. [3 ]
Garcia-Ispierto, I. [3 ]
Cabezon, O. [4 ]
Lopez-Gatius, F. [3 ]
Almeria, S. [1 ,2 ]
机构：
[1] UAB, Fac Vet, Dept Sanitat & Anat Anim, Cerdanyola Del Valles 08193, Spain
[2] UAB, IRTA, Ctr Recerca Sanitat Anim CReSA, Campus UAB, Cerdanyola Del Valles 08193, Spain
[3] Univ Lleida, Dept Prod Anim, Agrotecnio Ctr, Lleida, Spain
[4] UAB, SeFAS, Bellaterra, Spain
来源：
RESEARCH IN VETERINARY SCIENCE | 2016年 / 107卷
关键词：
Neospora caninum; Experimental and natural infection; Pregnant dams; Mid-gestation; Viable and aborted foetuses; IFN-gamma; IL-17A and IL-4 levels; HOST-PARASITE RELATIONSHIP; CD4(+) T-CELLS; IMMUNE-RESPONSES; BOVINE NEOSPOROSIS; PREGNANT CATTLE; INTERFERON-GAMMA; GENE-EXPRESSION; INTRACELLULAR MULTIPLICATION; EXPERIMENTAL CHALLENGE; DAIRY-COWS;
D O I：
10.1016/j.rvsc.2016.05.003
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
In the present study, IFN-gamma (Th1), IL-17 A (Th17) and IL-4 (Th2) concentrations in response to concanavalin (ConA) and Neospora caninum antigen (Nc-1) stimulation were determined in cultures of cells from control uninfected (n = 4), naturally N. caninum-infected (n = 3) and experimentally N. caninum-infected (n = 6) pregnant dams and their foetuses. Experimental animals were infected at 110 days of gestation and euthanized 6 weeks post-infection. In culture supernatants from the dams, significantly higher IFN-gamma and IL-4 levels were found in the experimentally-infected animals compared to the control or naturally-infected dams. However, among the experimentally-infected dams no significant differences in IFN-gamma production were observed regardless of the incidence of live or aborted/dead foetuses, though spleen cultures of dams carrying live foetuses showed the highest levels of IFN-gamma. IL-17A production was very low and occasional in the dams infected with N. caninum and did not seem to be a major regulator of IFN-gamma production in this model. Experimentally infected dams with live foetuses showed higher IL-4 levels and accordingly IFN-gamma/IL-4 ratios were significantly lower than ratios recorded for cows with aborted/dead foetuses. In the infected foetuses of these dams, only spleen cultures showed high levels of IFN-gamma and 1L-4 after Nc-1 antigen and ConA stimulation, respectively. No IL-17A was detected in the foetuses. As conclusion, although we could not clearly relate a protective immune response against N. caninum abortion only to IFN-gamma levels in cell cultures, our results highlight the important role of an inverse IFN-gamma/IL-4 balance in conferring protection against abortion induced by this parasite. (C) 2016 Elsevier Ltd. All rights reserved.
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页码：55 / 61
页数：7
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