Two new bioactive iridoids from Rothmannia wittii

The first reported study of the isolation and identification of compounds from the bark and fruit of Rothmannia wittii yielded two new iridoids, 6 beta-hydroxy-10-O-acetylgenipin (1) and 10-O-acetylmacrophyllide (2) together with six known iridoids; 6 beta-hydroxygenipin (3), genipin (4), garjasmine (5), cerbinal (6), and mixture of beta-gardiol (7) and alpha-gardiol (8); benzoic add (9); vanillic acid (10); and stigmasterol (11). Their structures were elucidated by spectroscopic methods. Iridoid 1 showed antimycobacterial activity against Mycobacterium tuberculosis with a MIC value of 12.50 mu g/mL. Iridoid 2 showed cytotoxicity against the NCI-H187 cancer cell line with an IC50 value of 6.82 mu g/mL. In addition, 2 and 5 exhibited weak cytotoxic activity against KB and MCF-7 cell lines, while 4 was active against the NCI-H187 cancer cell line. (C) 2016 Elsevisr B.V. All rights reserved.