Interaction of 9-Methoxyluminarine with Different G-Quadruplex Topologies: Fluorescence and Circular Dichroism Studies

被引：5

作者：

Nowak-Karnowska, Joanna ^{[1
]}

Gluszylska, Agata ^{[1
]}

Kosman, Joanna ^{[1
]}

Neunert, Grazyna ^{[2
]}

Dembska, Anna ^{[1
]}

机构：

[1] Adam Mickiewicz Univ, Fac Chem, Dept Bioanalyt Chem, Poznanskiego 8, PL-61614 Poznan, Poland

[2] Poznan Univ Life Sci, Fac Food Sci & Nutr, Dept Phys & Biophys, Wojska Polskiego 38-42, PL-60637 Poznan, Poland

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2021年 / 22卷 / 19期

关键词：

G-quadruplex; c-MYC; 9-methoxyluminarine; fluorescence; CD spectroscopy; DNA APTAMER; HUMAN TELOMERE; G-QUARTET; BINDING; RECOGNITION; THROMBIN; SEQUENCE; MOLECULE; MYC; VISUALIZATION;

D O I：

10.3390/ijms221910399

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interactions of G-quadruplexes of different topologies with highly fluorescent 9-methoxyluminarine ligand 9-MeLM were investigated by fluorescence and circular dichroism spectroscopy. The results showed that 9-methoxyluminarine was able to interact and did not destabilize any investigated molecular targets. The studied compound was selectively quenched by parallel c-MYC G-quadruplex DNA, whereas hybrid and antiparallel G4 topology caused only a negligible decrease in the fluorescence of the ligand. A high decrease of fluorescence of the ligand after binding with c-MYC G-quadruplex suggests that this molecule can be used as a selective probe for parallel G-quadruplexes.

引用

页数：16

共 79 条

[1] The Major G-Quadruplex Formed in the Human BCL-2 Proximal Promoter Adopts a Parallel Structure with a 13-nt Loop in K+ Solution [J].