Toward Clinical Proteomics on a Next-Generation Sequencing Platform

被引：15

作者：

Turner, Daniel J. ^{[2
]}

Tuytten, Robin ^{[1
]}

Janssen, Kris P. F. ^{[3
]}

Lammertyn, Jeroen ^{[3
]}

Wuyts, Jan ^{[1
]}

Pollet, Jeroen ^{[3
]}

Eyckerman, Sven ^{[1
]}

Brown, Clive ^{[1
]}

Kas, Koen ^{[1
]}

机构：

[1] Pronota Nv, B-9052 Zwijnaarde Ghent, Belgium

[2] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England

[3] Katholieke Univ Leuven, BIOSYST MeBioS, B-3001 Louvain, Belgium

来源：

ANALYTICAL CHEMISTRY | 2011年 / 83卷 / 03期

关键词：

CAPILLARY-ELECTROPHORESIS; AMPLIFICATION-FREE; SMART APTAMERS; DNA APTAMERS; NON-SELEX; RNA-SEQ; SELECTION; LIGANDS; MOLECULES; GENOME;

D O I：

10.1021/ac102666n

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report the first next generation sequencing (NGS) application to identify and quantify proteins. Customization of protein specific aptamers enabled direct conversion of serum protein information into NGS read outs. The intrinsic ability of aptamer sequencing to highly multiplex protein detection and quantification, together with the prospect of DNA sequencing further evolving into a commodity technology, could constitute the core of a novel, universal diagnostics paradigm.

引用

页码：666 / 670

页数：5

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