DNA damage in digestive gland and mantle tissue of the mussel Perna perna

被引：31
作者：
de Almeida, EA
Marques, SD
Klitzke, CF
Bainy, ACD
Medeiros, MHG
Di Mascio, P
Loureiro, APM
机构：
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05513970 Sao Paulo, Brazil
[2] Univ Fed Santa Catarina, Ctr Ciencias Biol, Dept Bioquim, Florianopolis, SC, Brazil
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 2003年 / 135卷 / 03期
基金：
巴西圣保罗研究基金会;
关键词：
DNA damage; 8-oxodGuo; etheno adduct; lipid peroxidation; mussel; oxidative stress; Perna Perna; mass spectrometry; HPLC;
D O I：
10.1016/S1532-0456(03)00117-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Data concerning the susceptibility of DNA to damage by reactive oxygen and nitrogen species and other endogenous compounds produced by physiological stress in marine organisms is lacking, especially in bivalve mollusks. In this article, we analyzed the background levels of lipid peroxidation (malondialdehyde, MDA), 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 1,N-2-etheno-2'-deoxyguanosine (1,N-2-epsilondGuo) in digestive gland and mantle tissue of mussels Perna perna collected at a cultivation zone in Florianopolis (Santa Catarina, Brazil). The present data point to the possibility of the use of both 8-oxodGuo and 1,N-2-epsilondGuo as complementary indicators of oxidative stress processes in mussels. A sensitive method coupling high performance liquid chromatography to mass spectrometry was applied for the detection of 1,N-2-epsilondGuo in mussel tissues. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码：295 / 303
页数：9
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