Protective effects of the aqueous extract of Scutellaria baicalensis against acrolein-induced oxidative stress in cultured human umbilical vein endothelial cells

被引：33

作者：

Zhang, Xing-Wei ^{[2
]}

Li, Wei-Fen ^{[2
]}

Li, Wei-Wei ^{[2
]}

Ren, Kan-Han ^{[2
]}

Fan, Chao-Ming ^{[2
]}

Chen, Ying-Ying ^{[1
]}

Shen, Yue-Liang ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Pathophysiol, Sch Med, Hangzhou 310058, Zhejiang, Peoples R China

[2] Hangzhou Normal Univ, Affiliated Hosp, Hangzhou, Zhejiang, Peoples R China

来源：

PHARMACEUTICAL BIOLOGY | 2011年 / 49卷 / 03期

基金：

中国国家自然科学基金;

关键词：

ISOLATED RAT-HEART; SEE VOL. 23; LIPID-PEROXIDATION; UP-REGULATION; GEORGI; GLUTATHIONE; EXPRESSION; DAMAGE; ANTIOXIDANT; PATHWAY;

D O I：

10.3109/13880209.2010.501803

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Objective: aEuro,This study was designed to determine the protective effects of the extract of SbG against the acrolein-induced oxidative stress in cultured human umbilical vein endothelial cells (HUVEC). Materials and methods: aEuro,The MTT reduction assay was employed to determine cell viability. The total cellular glutathione (GSH) level was detected using a colorimetric GSH assay kit. Cellular GSH production was conducted by detecting the mRNA expression levels of gamma gamma-glutamylcysteine ligase catalytic subunit and modifier subunit. Results: aEuro,Concentration-dependent cytotoxic effects of acrolein were observed while SbG could effectively protect the acrolein-induced oxidative damage. The protective mechanism was investigated, showing that the increased GSH content in the SbG-incubated HUVE cells was associated with the protective effects of SbG-treated cells. Further RT-PCR data confirmed the elevated mRNA expressions of GSH synthesis enzymes. Discussion and conclusion: aEuro,The current study strongly indicated that SbG could be a potential antioxidant against oxidative stress in treating cardiovascular diseases.

引用

页码：256 / 261

页数：6

共 44 条

[1] Garlic as an antioxidant: The good, the bad and the ugly [J].

Banerjee, SK ;

Mukherjee, PK ;

Maulik, SK .

PHYTOTHERAPY RESEARCH, 2003, 17 (02) :97-106

[2]

Breymann C, 2006, STEM CELL REV, V2, P87

[3] Expression, Purification and Characterization of C-FADD [J].

Chen, Yuan ;

Ma, Dingyuan ;

Huang, Qi-Lai ;

Zheng, Weijuan ;

Zhang, Jing ;

Shen, Yi ;

Li, Jiahuang ;

Dong, Wei ;

Lu, Min ;

Wang, Jin ;

Hua, Zi-Chun .

CELLULAR & MOLECULAR IMMUNOLOGY, 2009, 6 (04) :295-301

[4] Flavones from Scutellaria baicalensis Georgi attenuate apoptosis and protein oxidation in neuronal cell lines [J].

Choi, JG ;

Conrad, CC ;

Malakowsky, CA ;

Talent, JM ;

Yuan, CS ;

Gracy, RW .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2002, 1571 (03) :201-210

[5] Oxidation process of adrenaline in freshly isolated rat cardiomyocytes: Formation of adrenochrome, quinoproteins, and GSH adduct [J].

Costa, Vera Marisa ;

Silva, Renata ;

Ferreira, Luisa Maria ;

Branco, Paula Serio ;

Carvalho, Felix ;

Bastos, Maria Lourdes ;

Carvalho, Rui Albuquerque ;

Carvalho, Marcia ;

Remiao, Fernando .

CHEMICAL RESEARCH IN TOXICOLOGY, 2007, 20 (08) :1183-1191

[6] Endothelial function and vascular oxidative stress in long-lived GH/IGF-deficient Ames dwarf mice [J].

Csiszar, Anna ;

Labinskyy, Nazar ;

Perez, Viviana ;

Recchia, Fabio A. ;

Podlutsky, Andrej ;

Mukhopadhyay, Partha ;

Losonczy, Gyorgy ;

Pacher, Pal ;

Austad, Steven N. ;

Bartke, Andrzej ;

Ungvari, Zoltan .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2008, 295 (05) :H1882-H1894

[7] Analysis of the 4th Generation Scutellaria Baicalensis Georgi with Space Mutagenesis Via FTIR Spectroscopy [J].

Ding Xi-feng ;

Gao Hua-na ;

Guo Xi-hua ;

Wang Zhi-zhou ;

Yang La-hu ;

Guan Ying ;

Shi Jin-shan .

SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29 (05) :1286-1288

[8]

Erdbrugger W, 1989, Prog Clin Biol Res, V301, P389

[9] Protective effects of flavonoids in the roots of Scutellaria baicalensis Georgi against hydrogen peroxide-induced oxidative stress in HS-SY5Y cells [J].

Gao, ZH ;

Huang, KX ;

Xu, HB .

PHARMACOLOGICAL RESEARCH, 2001, 43 (02) :173-178

[10] RETRACTED: Cardioprotective effects of hydroxysafflor yellow A on diabetic cardiac insufficiency attributed to up-regulation of the expression of intracellular calcium handling proteins of sarcoplasmic reticulum in rats (Retracted article. See vol. 23, pg. 746, 2009) [J].

He, Haibo ;

Liu, Qiuju ;

Shi, Mengqiong ;

Zeng, Xiaowei ;

Yang, Jun ;

Wu, Limao ;

Li, Lianda .

PHYTOTHERAPY RESEARCH, 2008, 22 (08) :1107-1114

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