Diagnostic Significance of FNAB miRNA Expression in Papillary Thyroid Carcinoma

被引：1

作者：

Laukiene, Romena ^{[1
]}

Ambrozaityte, Laima ^{[1
]}

Cimbalistiene, Loreta ^{[1
]}

Utkus, Algirdas ^{[1
]}

Tamosiunas, Algirdas Edvardas ^{[2
]}

机构：

[1] Vilnius Univ, Fac Med, Inst Biomed Sci, Dept Human & Med Genet, Santariskiu 2, LT-08661 Vilnius, Lithuania

[2] Vilnius Univ, Fac Med, Inst Biomed Sci, Dept Radiol Nucl Med & Med Physis, Santariskiu 2, LT-08661 Vilnius, Lithuania

来源：

DIAGNOSTICS | 2022年 / 12卷 / 06期

关键词：

papillary thyroid carcinoma; miR125A; miR146B; miR221; miR4324; fine needle aspiration biopsy; LYMPH-NODE METASTASIS; ASSOCIATION GUIDELINES; MICRORNA EXPRESSION; CANCER; MUTATION; NODULES; SYSTEM;

D O I：

10.3390/diagnostics12061384

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The aim of the study was to evaluate the diagnostic utility of specific miRNAs in the preoperative assessment of thyroid nodules. One hundred and sixty thyroid fine needle aspiration biopsy (FNAB) samples with suspected thyroid carcinoma were collected. To detect the levels of miRNA expression in FNAB, next generation small RNA sequencing was performed in 60 samples. Based on the results obtained, three miRNAs (miR125A, miR200B, miR4324) were selected for further analysis. Based on the most frequently reported miRNAs in the literature associated with thyroid papillary carcinoma (PTC), two more miRNA (miR146B, miR221) were selected for further validation, using real-time reverse transcriptase polymerase chain reaction (RT-PCR) in 36 benign and 64 PTC samples. Expression of miR125A, miR146B, miR221, and miR4324 was significantly higher in patients with PTC compared with benign thyroid nodules (p < 0.05). miR125A and miR4324 were also significantly more highly expressed in patients with extrathyroidal tumor extension compared to those without extrathyroidal PTC extension (p < 0.001). We also found a significantly higher expression of miR221 (p = 0.043) in patients with multifocal carcinomas compared to patients with single foci carcinomas. This prospective study showed that the expression analysis of four miRNAs (miR125A, miR146B, miR221, and miR4324) improve accuracy of FNAB, which could allow a better pre-operative diagnostic and prognostic assessment of thyroid malignancies.

引用

页数：14

共 40 条

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