Real-Time In Situ Screening of Omega-7 Phospholipids in Marine Biological Resources Using an iKnife-Rapid-Evaporative-lonization-Mass-Spectrometry-Based Lipidomics Phenotype

被引：37

作者：

Song, Gongshuai ^{[1
]}

Wang, Qingcheng ^{[2
]}

Zhang, Mengna ^{[1
]}

Yang, Huijuan ^{[3
]}

Xie, Hujun ^{[1
]}

Zhao, Qiaoling ^{[4
]}

Zhu, Qinchao ^{[1
]}

Zhang, Xiaodi ^{[1
]}

Wang, Honghai ^{[1
]}

Wang, Pingya ^{[4
]}

Shen, Qing ^{[1
]}

机构：

[1] Zhejiang Gongshang Univ, Collaborat Innovat Ctr Seafood Deep Proc, Inst Seafood, Zhejiang Prov Joint Key Lab Aquat Prod Proc, Hangzhou 310012, Zhejiang, Peoples R China

[2] Hangzhou Yuhang Hosp Tradit Chinese Med, Dept Cardiol, Yuhang 311106, Zhejiang, Peoples R China

[3] China Jiliang Univ, Coll Standardizat, Hangzhou 310018, Zhejiang, Peoples R China

[4] Zhoushan Inst Calibrat & Testing Qual & Tech Supe, Zhoushan 316021, Zhejiang, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2021年 / 69卷 / 32期

基金：

国家重点研发计划;

关键词：

omega-7; phospholipids; iKnife REIMS; multivariate recognition analysis; lipidomics; marine resources; OXIDATION CHARACTERISTICS; FISH-OIL; LIPIDS;

D O I：

10.1021/acs.jafc.0c05442

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Omega-7 (n-7) phospholipids were bioactive substances in marine animals. In this study, a fast lipidomics phenotyping approach for real-time in situ screening of n-7 phospholipids in five kinds of economic seafood, salmon, prawn, bluefin tuna, hairtail, and butterfish, was established using iKnife rapid evaporative ionization mass spectrometry (REIMS). The n-7 phospholipids were structurally characterized and quantitatively analyzed, and the profiles were statistically analyzed by multivariate recognition analysis. It indicated that the difference of n-7 phospholipids in seafood samples was significant (p < 0.05), with R-2 (cum) and Q(2) (cum) values of >0.9. The proportion of n-7 phospholipids in salmon was the highest (20.43%), followed by bluefin tuna, prawn, hairtail, and butterfish. The ions of m/z 742.54 (PC 16:1-18:1), 768.55 (PC 16:1-20:2), 697.48 (PE 16:1-18:1), and 699.48 (PE 16:1 18:0) were the main n-7 phospholipids. The effectiveness of iKnife REIMS was further verified by hydrophilic interaction chromatography mass spectrometry and gas chromatography. The results demonstrated that proposed iKnife REIMS was an excellent technique for front-line screening of n-7 phospholipids in a large variety of marine biological resources.

引用

页码：9004 / 9011

页数：8

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