The modification of the thrombin generation assay for the clinical assessment of hypercoagulability in patients receiving heparin therapy

被引：5
作者：
Benoit, Remi [1 ]
Nougier, Christophe [1 ]
Desmurs-Clavel, Helene [2 ,3 ]
Simon, Marie [3 ,4 ]
Dargaud, Yesim [1 ,3 ,5 ,6 ]
机构：
[1] Hosp Civils Lyon, Grp Hosp Est, Lab Hematol, Lyon, France
[2] Hop Edouard Herriot, Serv Med Interne, Lyon, France
[3] Grp Etud Multidisciplinaire Malad Thrombot, GEMMAT, Lyon, France
[4] Hop Edouard Herriot, Serv Med Intens Reanimat, Lyon, France
[5] Hop Cardiovasc & Pneumol Louis Pradel, Unite Hemostase Clin, 28 Av Doyen Lepine, F-69500 Lyon, France
[6] Univ Claude Bernard Lyon 1, UR4609 Hemostase & Thrombose, Lyon, France
来源：
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY | 2022年 / 44卷 / 02期
关键词：
heparin; heparinase; polybrene; protamine sulfate; rhodamine; thrombin generation assay; RECURRENT VENOUS THROMBOSIS; IN-VITRO; RISK;
D O I：
10.1111/ijlh.13735
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Background Heparin diminishes thrombin generation (TG) because it decreases the survival time of thrombin in plasma. Under heparin therapy, the TG curve therefore does not reflect the true hemostatic status of the patient. Aim We investigated how far the in vitro addition of a heparin antagonist can restore the underlying TG capacity. Materials & Methods Five different heparin antagonists were tested: polybrene, protamine sulfate, heparinase type 1, heparinase HEP-TEM, and (Z-GGR)(2)-rhodamine (P2Rho). Results and Conclusion Polybrene, P2Rho, and heparinase HEP-TEM effectively neutralized heparin at prophylactic and therapeutical doses of both low molecular weight and unfractionated heparin. The advantages and limits of each molecule and the most favorable combinations of TG-trigger and antagonist are discussed.
引用
收藏
页码：371 / 378
页数：8
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