Development and efficacy of a novel live-attenuated QX-like nephropathogenic infectious bronchitis virus vaccine in China

被引：54

作者：

Feng, Keyu ^{[1
,2
]}

Xue, Yu ^{[1
,4
]}

Wang, Jinglan ^{[6
]}

Chen, Weiguo ^{[1
,3
]}

Chen, Feng ^{[1
,3
]}

Bi, Yingzuo ^{[1
,2
,3
]}

Xie, Qingmei ^{[1
,2
,3
,5
]}

机构：

[1] South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China

[2] Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China

[3] Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Guangdong, Peoples R China

[4] Guangdong Wens Foodstuff Grp Co Ltd, Guangdong Enterprise Key Lab Anim Hlth & Environm, Yunfu 527439, Peoples R China

[5] South China Collaborat Innovat Ctr Poultry Dis Co, Guangzhou 510640, Guangdong, Peoples R China

[6] Langfang Acad Agr & Forestry Sci, Langfang 065000, Peoples R China

来源：

VACCINE | 2015年 / 33卷 / 09期

关键词：

Live-attenuated vaccine strain; QX-like; Nephropathogenic; Infectious bronchitis virus; TRANSMISSIBLE GASTROENTERITIS CORONAVIRUS; SPIKE PROTEIN; GENE; SEQUENCE; S1; EPIDEMIOLOGY; DETERMINANT; GENOME; STRAIN; PATHOGENICITY;

D O I：

10.1016/j.vaccine.2015.01.036

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In this study, we attenuated a Chinese QX-like nephropathogenic infectious bronchitis virus (IBV) strain, YX10, by passaging through fertilized chicken eggs. The 90th passage strain (YX10p90) was selected as the live-attenuated vaccine candidate strain. YX10p90 was found to be safe in 7-day-old specific pathogen free chickens without induction of morbidity or mortality. YX10p90 provided nearly complete protection against QX-like (CH I genotype) strains and partial protection against other two major Chinese genotype strains. YX10p90 also showed no reversion to virulence after five back passages in chickens. An IBV polyvalent vaccine containing YX10p90 was developed and showed that it could provide better protection against major Chinese IBV virulent strains than commercial polyvalent vaccines. In addition, the complete genome sequence of YX10p90 was sequenced. Multiple-sequence alignments identified 38 nucleotide substitutions in the whole genome which resulted in 26 amino acid substitutions and a 110-bp deletion in the 3' untranslated region. In conclusion, the attenuated YX10p90 strain exhibited a fine balance between attenuation and immunogenicity, and should be considered as a candidate vaccine to prevent infection of Chinese QX-like nephropathogenic IBV. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：1113 / 1120

页数：8

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