Ratiometric Emission Fluorescent pH Probe for Imaging of Living Cells in Extreme Acidity

被引：121

作者：

Niu, Weifen ^{[1
,2
]}

Fan, Li ^{[1
]}

Nan, Ming ^{[1
]}

Li, Zengbo ^{[1
]}

Lu, Dongtao ^{[1
]}

Wong, Man Shing ^{[1
,3
,4
]}

Shuang, Shaomin ^{[1
]}

Dong, Chuan ^{[1
]}

机构：

[1] Shanxi Univ, Coll Chem & Chem Engn, Inst Environm Sci, Taiyuan 030006, Peoples R China

[2] Yangtze Normal Univ, Dept Chem & Chem Engn, Chongqing 408100, Fuling, Peoples R China

[3] Hong Kong Baptist Univ, Dept Chem, Hong Kong, Hong Kong, Peoples R China

[4] Hong Kong Baptist Univ, Inst Adv Mat, Hong Kong, Hong Kong, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2015年 / 87卷 / 05期

基金：

中国国家自然科学基金;

关键词：

LYSOSOMAL PH; LIVE CELLS; SENSOR; HOMEOSTASIS; FLUOROPHORE;

D O I：

10.1021/ac504109h

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel ratiometric emission fluorescent probe, 1,1-dimethyl-2-[2-(quinolin-4-yl)vinyl]-1H-benzo[e]indole (QVBI), is facilely synthesized via ethylene bridging of benzoindole and quinoline. The probe exhibits ratiometric fluorescence emission (F-522nm/F-630nm) characteristics with pKa 3.27 and linear response to extreme-acidity range of 3.8-2.0. Also, its high fluorescence quantum yield (Phi = 0.89) and large Stokes shift (110 nm) are favorable. Moreover, QVBI possesses highly selective response to H+ over metal ions and some bioactive molecules, good photostability, and excellent reversibility. The probe has excellent cell membrane permeability and is further applied successfully to monitor pH fluctuations in live cells and imaging extreme acidity in Escherichia coli cells without influence of autofluorescence and native cellular species in biological systems.

引用

页码：2788 / 2793

页数：6

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