Effects of various steroids on platelet-derived endothelial cell growth factor (PD-ECGF) and its mRNA expression in uterine endometrial cancer cells

被引：4

作者：

Aoki, I ^{[1
]}

Fujimoto, J ^{[1
]}

Tamaya, T ^{[1
]}

机构：

[1] Gifu Univ, Sch Med, Dept Obstet & Gynecol, Gifu 5008705, Japan

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2003年 / 84卷 / 2-3期

关键词：

PD-ECGF; angiogenesis; endometrial cancer cell; steroids;

D O I：

10.1016/S0960-0760(03)00031-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Progestins diminish the estrogen-induced angiogenic potential related to basic fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF) in uterine endometrial cancer cells. This led us to study the effect of various steroids on the expression of platelet-derived endothelial cell growth factor (PD-ECGF) as the other pertinent angiogenic factor in well-differentiated uterine endometrial cancer cell line Ishikawa. In Ishikawa cells, estradiol induced the expression of PD-ECGF and its mRNA. The estrogen-induced expression was increased approximately two-fold by progesterone and by its metabolite, 17alpha-hydroxyprogesterone, but not by medroxyprogesterone acetate (MPA). Therefore, progesterone and 17alpha-hydroxyprogesterone as endogenous steroids might induce PD-ECGF-related angiogenic potential in uterine endometrial cancer cells, but not MPA as a synthetic steroid. In conclusion, the failure of PD-ECGF induction by MPA might be the great merit of anti-angiogenic treatment with MPA for uterine endometrial cancers. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：217 / 222

页数：6

共 32 条

[1] THE COMPLETE SEQUENCE OF A FULL LENGTH CDNA FOR HUMAN-LIVER GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - EVIDENCE FOR MULTIPLE MESSENGER-RNA SPECIES [J].

ARCARI, P ;

MARTINELLI, R ;

SALVATORE, F .

NUCLEIC ACIDS RESEARCH, 1984, 12 (23) :9179-9189

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[4] ANGIOGENESIS INVITRO [J].

FOLKMAN, J ;

HAUDENSCHILD, C .

NATURE, 1980, 288 (5791) :551-556

[5] TUMOR ANGIOGENESIS [J].

FOLKMAN, J .

ADVANCES IN CANCER RESEARCH, 1985, 43 :175-203

[6] ANGIOSTATIC STEROIDS - METHOD OF DISCOVERY AND MECHANISM OF ACTION [J].

FOLKMAN, J ;

INGBER, DE .

ANNALS OF SURGERY, 1987, 206 (03) :374-383

[7] Expression of platelet-derived endothelial cell growth factor (PD-ECGF) and its mRNA in ovarian cancers [J].

Fujimoto, J ;

Ichigo, S ;

Sakaguchi, H ;

Hirose, R ;

Tamaya, T .

CANCER LETTERS, 1998, 126 (01) :83-88

[8] Expression of platelet-derived endothelial cell growth factor (PD-ECGF) and its mRNA in uterine cervical cancers [J].

Fujimoto, J ;

Sakaguchi, H ;

Hirose, R ;

Ichigo, S ;

Tamaya, T .

BRITISH JOURNAL OF CANCER, 1999, 79 (7-8) :1249-1254

[9]

FUJIMOTO J, 1989, INVAS METAST, V9, P269

[10] Expression of platelet-derived endothelial cell growth factor and its mRNA in uterine endometrium during the menstrual cycle [J].

Fujimoto, J ;

Ichigo, S ;

Sakaguchi, H ;

Hirose, R ;

Tamaya, T .

MOLECULAR HUMAN REPRODUCTION, 1998, 4 (05) :509-513

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