Detection of Arginine by AuNPs/CQDs Nanoprobes Based on Fluorescence Resonance Energy Transfer Effect

被引：13

作者：

Zou Xiao-Bo ^{[1
]}

Shi Yong-Qiang ^{[1
]}

Zheng Yue ^{[2
]}

Shi Ji-Yong ^{[1
]}

Hu Xue-Tao ^{[1
]}

Jiang Cai-Ping ^{[1
]}

Huang Xiao-Wei ^{[1
]}

Xu Yi-Wei ^{[1
]}

机构：

[1] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China

[2] Chinese Acad Agr Mechanizat Sci, Beijing 100083, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2018年 / 46卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Arginine; fluorescent probe; Fluorescence resonance energy transfer; Carbon quantum dots; Gold nanoparticles; CARBON QUANTUM DOTS; COLORIMETRIC DETECTION; VISUAL DETECTION; FRET SYSTEM; SENSOR; ASSAY; COMPOSITE; COMPLEX;

D O I：

10.11895/j.issn.0253.3820.181096

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel method for rapid detection of arginine based on fluorescence resonance energy transfer effect ( FRET) between carbon quantum dots (CQDs) and gold nanoparticles (AuNPs) was developed. Firstly, the CQDs with excellent fluorescence properties were synthesized by one-step microwave assisted method. The AuNPs/CQDs composites were characterized and their quenching mechanism was analyzed. Then the amount of AuNPs/CQDs, the pH value and the reaction time were optimal. Under the optimum conditions, the fluorescence system was used to detect the content of arginine, showing a good linear relationship (R-2 = 0.993) between fluorescence intensity and concentration of arginine in the range of 0.1 - 10.0 mu mol/L, and the detection limit was 5.8 nmol/L. Finally, the content of arginine in grape juice was determined by this method with recoveries of 105.4% - 110.8%, which indicated that the proposed FRET system had the potential for practical detection of arginine in fruit juice.

引用

页码：960 / 968

页数：9

共 37 条

[31] Highly sensitive and selective aptasensor for detection of adenosine based on fluorescence resonance energy transfer from carbon dots to nano-graphite [J].

Wang, Xu ;

Xu, Guanhong ;

Wei, Fangdi ;

Ma, Yunsu ;

Ma, Yujie ;

Song, Yueyue ;

Cen, Yao ;

Hu, Qin .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2017, 508 :455-461

[32] Carbon quantum dots: synthesis, properties and applications [J].

Wang, Youfu ;

Hu, Aiguo .

JOURNAL OF MATERIALS CHEMISTRY C, 2014, 2 (34) :6921-6939

[33] Using FT-NIR spectroscopy technique to determine arginine content in fermented Cordyceps sinensis mycelium [J].

Xie, Chuanqi ;

Xu, Ning ;

Shao, Yongni ;

He, Yong .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2015, 149 :971-977

[34] A Rapid Colorimetric Detection of Melamine in Raw Milk by Unmodified Gold Nanoparticles [J].

Xing, Hai-bo ;

Wu, Yuan-gen ;

Zhan, Shen-shan ;

Zhou, Pei .

FOOD ANALYTICAL METHODS, 2013, 6 (05) :1441-1447

[35] Green Synthesis of Fluorescent Carbon Quantum Dots for Detection of Hg2+ [J].

Xu Yue ;

Tang Chun-Jing ;

Huang Hong ;

Sun Chao-Qun ;

Zhang Ya-Kun ;

Ye Qun-Feng ;

Wang Ai-Jun .

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2014, 42 (09) :1252-1258

[36] Near-infrared ratiometric fluorescent detection of arginine in lysosome with a new hemicyanine derivative [J].

Yu, Mingming ;

Du, Weiwei ;

Li, Haixia ;

Zhang, Hongyan ;

Li, Zhanxian .

BIOSENSORS & BIOELECTRONICS, 2017, 92 :385-389

[37] Selective detection of zwitterionic arginine with a new Zn(II)-terpyridine complex: potential application in protein labeling and determination [J].

Zhou, Xin ;

Jin, Xuejun ;

Li, Donghao ;

Wu, Xue .

CHEMICAL COMMUNICATIONS, 2011, 47 (13) :3921-3923

← 1 2 3 4 →