Characterization, Classification and Authentication of Spanish Blossom and Honeydew Honeys by Non-Targeted HPLC-UV and Off-Line SPE HPLC-UV Polyphenolic Fingerprinting Strategies

被引:18
作者
Garcia-Seval, Victor [1 ]
Martinez-Alfaro, Claudia [1 ]
Saurina, Javier [1 ,2 ]
Nunez, Oscar [1 ,2 ]
Sentellas, Sonia [1 ,2 ,3 ]
机构
[1] Univ Barcelona, Dept Chem Engn & Analyt Chem, Marti & Franques 1-11, E-08028 Barcelona, Spain
[2] Univ Barcelona, Res Inst Food Nutr & Food Safety, Av Prat Riba 171,Edifici Recerca Gaudi, E-08921 Barcelona, Spain
[3] Generalitat Catalunya, Rambla de Catalunya 19-21, E-08007 Barcelona, Spain
关键词
blossom honeys; honeydew honeys; HPLC-UV; fingerprinting; chemometrics; DIODE-ARRAY DETECTION; LIQUID-CHROMATOGRAPHY; PHENOLIC-COMPOUNDS; GEOGRAPHIC ORIGINS; SYRUP; SPECTROSCOPY; ADULTERATION; MARKERS;
D O I
10.3390/foods11152345
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Honey is a highly consumed natural product produced by bees which is susceptible to fraudulent practices, some of them regarding its botanical origin. Two HPLC-UV non-targeted fingerprinting approaches were evaluated in this work to address honey characterization, classification, and authentication based on honey botanical variety. The first method used no sample treatment and a universal reversed-phase chromatographic separation. On the contrary, the second method was based on an off-line SPE preconcentration method, optimized for the isolation and extraction of polyphenolic compounds, and a reversed-phase chromatographic separation optimized for polyphenols as well. For the off-line SPE method, the use of HLB (3 mL, 60 mg) cartridges, and 6 mL of methanol as eluent, allowed to achieve acceptable recoveries for the selected polyphenols. The obtained HPLC-UV fingerprints were subjected to an exploratory principal component analysis (PCA) and a classificatory partial least squares-discriminant analysis (PLS-DA) to evaluate their viability as sample chemical descriptors for authentication purposes. Both HPLC-UV fingerprints resulted to be appropriate to discriminate between blossom honeys and honeydew honeys. However, a superior performance was accomplished with off-line SPE HPLC-UV polyphenolic fingerprints, being able to differentiate among the different blossom honey samples under the study (orange/lemon blossom, rosemary, thyme, eucalyptus, and heather). In general, this work demonstrated the feasibility of HPLC-UV fingerprints, especially those obtained after off-line SPE polyphenolic isolation and extraction, to be employed as honey chemical descriptors to address the characterization and classification of honey samples according to their botanical origin.
引用
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页数:20
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