Recovery of β-galactosidase produced by Kluyveromyces lactis by ion-exchange chromatography: Influence of pH and ionic strength parameters

被引：4
作者：
de Carvalho, Catherine T. [1 ]
de Oliveira Junior, Sergio D. [1 ]
de Brito Lima, Wildson B. [1 ]
Macedo de Medeiros, Fabio G. [1 ]
de Sa Leitao, Ana Laura O. [1 ]
Dantas, Julia M. M. [1 ]
dos Santos, Everaldo S. [1 ]
de Macedo, Gorete R. [1 ]
de Sousa Junior, Francisco C. [2 ]
机构：
[1] Univ Fed Rio Grande do Norte, Dept Engn Quim, UFRN, Av Senador Salgado Filho, BR-59078970 Natal, RN, Brazil
[2] Univ Fed Rio Grande do Norte, Dept Farm, UFRN, Av Gal Gustavo Cordeiro Faria S-N, BR-59012570 Natal, RN, Brazil
来源：
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS | 2022年 / 94卷 / 01期
关键词：
beta-galactosidase; adsorption; Kluyveromyces lactis; ion-exchange chromatography; recovery; CHEESE WHEY; 503; ANTIGEN; PURIFICATION; ADSORPTION; HYDROLYSIS; LACTOSE;
D O I：
10.1590/0001-3765202220200752
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The use of beta-galactosidase in food products has been a major focus of the industry. Therefore, the development of efficient and inexpensive methodologies to purify it is essential. Thus, this study aimed to recover the enzyme beta-galactosidase (beta-gal) by ion-exchange chromatography in a fixed-bed column. Batch adsorption tests were performed using four types of adsorbents. The beta-gal adsorption capacity in batch mode using Streamline DEAE resin presented the best performance, with a retention capacity of 18.77 +/- 0.14 U/g at pH 6.0. A 2(2) experimental design was applied to optimize the beta-gal recovery using an AKTA Start system, evaluating the ionic strength and the pH as process parameters. The results showed that ionic strength exerted a greater influence on fold purification (FP). The beta-gal fraction in elution using 0.1-0.4 M of NaCl showed a yield of 51.65 +/- 0.17% and FP of 2.00 +/- 0.43. Electrophoresis confirmed the beta-gal recovery, where an evident band with a molecular weight between 60 and 120 kDa was observed. These results point to the recovery of a stable beta-gal of K. lactis with potential industrial applications.
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页数：12
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