Fiber Confocal Back-Scattering Micro-Spectral Analysis for Single Cell

被引：2
作者：
Wang, C. [2 ]
Li, Y. [1 ,3 ]
Liu, G. [3 ,4 ]
Ma, J. [2 ]
Li, Z. [2 ]
Wei, X. [1 ,3 ]
机构：
[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
[2] Univ Shanghai Sci & Technol, Lab Med Opt & Optometry, Sch Med Instrument & Food Engn, Shanghai 200093, Peoples R China
[3] Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China
[4] Fudan Univ, Shanghai Med Sch, Shanghai 200032, Peoples R China
来源：
TECHNOLOGY IN CANCER RESEARCH & TREATMENT | 2011年 / 10卷 / 05期
基金：
美国国家科学基金会;
关键词：
Back-scattering; Micro-spectral analysis; Cancer cell; Fiber-confocal; ELASTIC LIGHT-SCATTERING; BIOLOGICAL CELLS; REFLECTANCE; SPECTROSCOPY; ABSORPTION; ORGANELLES; DIAGNOSIS; TISSUE;
D O I：
10.7785/tcrt.2012.500222
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
A fiber confocal back scattering micro-spectrometer (FCBS) was established, which combined fiber confocal microscopy with light scattering spectroscopy (LSS) for early diagnosis of the cancer cell at cellular level. An adherent monolayer human normal gastric epithelium line GES-1 and a carcinoma cell line NCI-N87 as well as a normal liver cell line L02 and a high-metastatic-potential hepatocellular carcinoma cell line HCC-LM3 were measured respectively. The spectral results showed that micro-back-scattering intensity from GES-1 cell and L02 cell possessed interesting oscillations in contrast to NCI-N87 and HCC-LM3 cells. There was significant difference between the spectra of the normal and the cancer cells (p < 0.001). This demonstrates that the FCBS system here is able to distinguish dysplastic cells from normal cells at cellular level.
引用
收藏
页码：457 / 463
页数：7
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