Homologous prime-boost with Zika virus envelope protein and poly (I:C) induces robust specific humoral and cellular immune responses

被引：17

作者：

Amaral, Marcelo Pires ^{[1
]}

Apostolico, Juliana de Souza ^{[1
,2
]}

Tomita, Nadia ^{[1
]}

Coirada, Fernanda Caroline ^{[1
]}

Santos Lunardelli, Victoria Alves ^{[1
,2
]}

Fernandes, Edgar Ruz ^{[1
]}

Santos Souza, Higo Fernando ^{[3
]}

Astray, Renato Mancini ^{[4
]}

Boscardin, Silvia Beatriz ^{[2
,3
]}

Rosa, Daniela Santoro ^{[1
,2
]}

机构：

[1] Fed Univ Sao Paulo UNIFESP EPM, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil

[2] INCT, Inst Invest Immunol 3, Sao Paulo, Brazil

[3] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Sao Paulo, Brazil

[4] Butantan Inst, Viral Immunol Lab, Sao Paulo, Brazil

来源：

VACCINE | 2020年 / 38卷 / 20期

基金：

巴西圣保罗研究基金会;

关键词：

Subunit vaccine; DNA vaccine; Prime-boost; Adjuvant; Envelope; Zika virus; DNA VACCINE; INFECTION; CELLS;

D O I：

10.1016/j.vaccine.2020.03.037

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The recent outbreaks of Zika virus (ZIKV) infection and the potential association with Guillain-Barre syndrome in adults and with congenital abnormalities have highlighted the urgency for an effective vaccine. The ZIKV Envelope glycoprotein (E-ZIKV) is the most abundant protein on the virus surface, and has been evaluated together with the pre-membrane protein (prM) of the viral coat as a vaccine candidate in clinical trials. In this study, we performed a head-to-head comparison of the immune response induced by different E-ZIKV-based vaccine candidates in mice. We compared different platforms (DNA, recombinant protein), adjuvants (poly (I:C), CpG ODN 1826) and immunization strategies (homologous, heterologous). The hierarchy of adjuvant potency showed that poly (I:C) was a superior adjuvant than CpG ODN. While poly (I:C) assisted immunization reached a plateau in antibody titers after two doses, the CpG ODN group required an extra immunization dose. Besides, the administration of poly (I:C) induced higher E-ZIKV-specific cellular immune responses than CpG ODN. We also show that immunization with homologous prime-boost EZIKV protein + poly (I:C) regimen induced a more robust humoral response than homologous DNA (pVAX-E-ZIKV) or heterologous regimens (DNA/protein or protein/DNA). A detailed analysis of cellular immune responses revealed that homologous (E-ZIKV + poly (I:C)) and heterologous (pVAX-E-ZIKV/E-ZIKV + poly (I:C)) prime-boost regimens induced the highest magnitude of IFN-gamma secreting cells and cytokine-producing CD4(+) T cells. Overall, our data demonstrate that homologous E-ZIKV + poly (I:C) prime-boost immunization is sufficient to induce more robust specific-E-ZIKV humoral and cellular immune responses than the other strategies that contemplate homologous DNA (pVAX-E-ZIKV) or heterologous (pVAX-E-ZIKV/E-ZIKV + poly (I:C), and vice-versa) immunizations. (C) 2020 Elsevier Ltd. All rights reserved.

引用

页码：3653 / 3664

页数：12

共 61 条

[1] Durability and correlates of vaccine protection against Zika virus in rhesus monkeys [J].

Abbink, Peter ;

Larocca, Rafael A. ;

Visitsunthorn, Kittipos ;

Boyd, Michael ;

De la Barrera, Rafael A. ;

Gromowski, Gregory D. ;

Kirilova, Marinela ;

Peterson, Rebecca ;

Li, Zhenfeng ;

Nanayakkara, Ovini ;

Nityanandam, Ramya ;

Mercado, Noe B. ;

Borducchi, Erica N. ;

Chandrashekar, Abishek ;

Jetton, David ;

Mojta, Shanell ;

Gandhi, Priya ;

LeSuer, Jake ;

Khatiwada, Shreeya ;

Lewis, Mark G. ;

Modjarrad, Kayvon ;

Jarman, Richard G. ;

Eckels, Kenneth H. ;

Thomas, Stephen J. ;

Michael, Nelson L. ;

Barouch, Dan H. .

SCIENCE TRANSLATIONAL MEDICINE, 2017, 9 (420)

[2] Protective efficacy of multiple vaccine platforms against Zika virus challenge in rhesus monkeys [J].

Abbink, Peter ;

Larocca, Rafael A. ;

De La Barrera, Rafael A. ;

Bricault, Christine A. ;

Moseley, Edward T. ;

Boyd, Michael ;

Kirilova, Marinela ;

Li, Zhenfeng ;

Ng'ang'a, David ;

Nanayakkara, Ovini ;

Nityanandam, Ramya ;

Mercado, Noe B. ;

Borducchi, Erica N. ;

Agarwal, Arshi ;

Brinkman, Amanda L. ;

Cabral, Crystal ;

Chandrashekar, Abishek ;

Giglio, Patricia B. ;

Jetton, David ;

Jimenez, Jessica ;

Lee, Benjamin C. ;

Mojta, Shanell ;

Molloy, Katherine ;

Shetty, Mayuri ;

Neubauer, George H. ;

Stephenson, Kathryn E. ;

Peron, Jean Pierre S. ;

Zanotto, Paolo M. de A. ;

Misamore, Johnathan ;

Finneyfrock, Brad ;

Lewis, Mark G. ;

Alter, Galit ;

Modjarrad, Kayvon ;

Jarman, Richard G. ;

Eckels, Kenneth H. ;

Michael, Nelson L. ;

Thomas, Stephen J. ;

Barouch, Dan H. .

SCIENCE, 2016, 353 (6304) :1129-1132

[3] The Yellow Fever Virus Vaccine Induces a Broad and Polyfunctional Human Memory CD8+ T Cell Response [J].

Akondy, Rama S. ;

Monson, Nathan D. ;

Miller, Joseph D. ;

Edupuganti, Srilatha ;

Teuwen, Dirk ;

Wu, Hong ;

Quyyumi, Farah ;

Garg, Seema ;

Altman, John D. ;

Del Rio, Carlos ;

Keyserling, Harry L. ;

Ploss, Alexander ;

Rice, Charles M. ;

Orenstein, Walter A. ;

Mulligan, Mark J. ;

Ahmed, Rafi .

JOURNAL OF IMMUNOLOGY, 2009, 183 (12) :7919-7930

[4] Poly(I:C) as cancer vaccine adjuvant: Knocking on the door of medical breakthroughs [J].

Ammi, Rachid ;

De Waele, Jorrit ;

Willemen, Yannick ;

Van Brussel, Ilse ;

Schrijvers, Dorien M. ;

Lion, Eva ;

Smits, Evelien L. J. .

PHARMACOLOGY & THERAPEUTICS, 2015, 146 :120-131

[5]

[Anonymous], J VACC VACC

[6] Dendritic Cell Targeting Effectively Boosts T Cell Responses Elicited by an HIV Multiepitope DNA Vaccine [J].

Apostolico, Juliana de Souza ;

Santos Lunardelli, Victoria Alves ;

Yamamoto, Marcio Massao ;

Santos Souza, Higo Fernando ;

Cunha-Neto, Edecio ;

Boscardin, Silvia Beatriz ;

Rosa, Daniela Santoro .

FRONTIERS IN IMMUNOLOGY, 2017, 8

[7] Adjuvants: Classification, Modus Operandi, and Licensing [J].

Apostolico, Juliana de Souza ;

Santos Lunardelli, Victoria Alves ;

Coirada, Fernanda Caroline ;

Boscardin, Silvia Beatriz ;

Rosa, Daniela Santoro .

JOURNAL OF IMMUNOLOGY RESEARCH, 2016, 2016

[8] Association between microcephaly, Zika virus infection, and other risk factors in Brazil: final report of a case-control study [J].

Barreto de Araujo, Thalia Velho ;

de Alencar Ximenes, Ricardo Arraes ;

Miranda-Filho, Democrito de Barros ;

Souza, Wayner Vieira ;

Montarroyos, Ulisses Ramos ;

Lopes de Melo, Ana Paula ;

Valongueiro, Sandra ;

Pessoa Militao de Albuquerque, Maria de Fatima ;

Braga, Cynthia ;

Brandao Filho, Sinval Pinto ;

Cordeiro, Marli Tenorio ;

Vazquez, Enrique ;

Souza Cruz, Danielle di Cavalcanti ;

Pessanha Henriques, Claudio Maierovitch ;

Albuquerque Bezerra, Luciana Caroline ;

da Silva Castanha, Priscila Mayrelle ;

Dhalia, Rafael ;

Azevedo Marques-Junior, Ernesto Torres ;

Turchi Martelli, Celina Maria ;

Rodrigues, Laura Cunha .

LANCET INFECTIOUS DISEASES, 2018, 18 (03) :328-336

[9]

Baud D, 2017, LANCET, V390, P2099, DOI [10.1016/S0140-6736(17)31450-2, 10.1016/s0140-6736(17)31450-2]

[10] Rapid travel to a Zika vaccine: are we heading towards success or more questions? [J].

Britto, Carl ;

Dold, Christina ;

Reyes-Sandoval, Arturo ;

Rollier, Christine S. .

EXPERT OPINION ON BIOLOGICAL THERAPY, 2018, 18 (11) :1171-1179

← 1 2 3 4 5 6 7 →