Evaluation of the impact of freezing preparation techniques on the characterisation of alginate hydrogels by cryo-SEM

被引：118

作者：

Aston, Robyn ^{[1
]}

Sewell, Kim ^{[2
]}

Klein, Travis ^{[3
]}

Lawrie, Gwen ^{[1
]}

Grondahl, Lisbeth ^{[1
]}

机构：

[1] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia

[2] Univ Queensland, CMM, Brisbane, Qld 4072, Australia

[3] QUT, Inst Hlth & Biomed Innovat, Brisbane, Qld 4059, Australia

来源：

EUROPEAN POLYMER JOURNAL | 2016年 / 82卷

关键词：

Hydrogel; Cryo-SEM; Structural characterisation; Cryo-fixation; Pore size; TEMPERATURE ELECTRON-MICROSCOPY; POLYSACCHARIDE ULTRASTRUCTURES; SCAFFOLDS; CHITOSAN; CRYOPRESERVATION; VITRIFICATION;

D O I：

10.1016/j.eurpolymj.2016.06.025

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The structural characterisation of high water content materials such as hydrogels is challenging due to the inherent properties of water that are dependent on conditions of temperature and pressure. Cryo-scanning electron microscopy (cryo-SEM) has potential to offer the most authentic insights into the native structure of polysaccharide hydrogels, due to the ability to freeze the sample under conditions where transparent vitreous ice is formed. The advantages, challenges and constraints of cryo-SEM in characterising the porous matrix of alginate hydrogels are considered in this tutorial paper through examples from our own studies. Recommendations are proposed for the minimum methodological information that is required from researchers as part of the publication of their studies of hydrogel samples to enable critical appraisal of their cryo-SEM images. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：1 / 15

页数：15

共 52 条

[1] Hydrogel: Preparation, characterization, and applications: A review [J].

Ahmed, Enas M. .

JOURNAL OF ADVANCED RESEARCH, 2015, 6 (02) :105-121

[2] Microstructural studies of probiotic bacteria-loaded alginate microcapsules using standard electron microscopy techniques and anhydrous fixation [J].

Allan-Wojtas, P. ;

Hansen, L. Truelstrup ;

Paulson, A. T. .

LWT-FOOD SCIENCE AND TECHNOLOGY, 2008, 41 (01) :101-108

[3]

[Anonymous], 2009, HDB SAMPLE PREPARATI, DOI DOI 10.1007/978-0-387-85731-2

[4]

[Anonymous], ELECT MICROSCOPY PLA

[5]

Apkarian R.P., 2005, MICROS MICROANAL, V11, P1088, DOI [10.1017/S1431927605500163, DOI 10.1017/S1431927605500163]

[6] Preparation of Chitosan Nanocompositeswith a Macroporous Structure by Unidirectional Freezing and Subsequent Freeze-Drying [J].

Aranaz, Inmaculada ;

Gutierrez, Maria C. ;

Luisa Ferrer, Maria ;

del Monte, Francisco .

MARINE DRUGS, 2014, 12 (11) :5619-5642

[7]

Bachmann L., 1987, CRYOTECHNIQUES BIOL, P192

[8]

Bachmann L., 1987, Cryotechniques in Biological Electron Microscopy, P3, DOI DOI 10.1007/978-3-642-72815-0_1

[9]

CHAPMAN RL, 1986, ULTRASTRUCTURE TECHN, P213

[10] HIGH-PRESSURE FREEZING FOR THE PRESERVATION OF BIOLOGICAL STRUCTURE - THEORY AND PRACTICE [J].

DAHL, R ;

STAEHELIN, LA .

JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE, 1989, 13 (03) :165-174

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