Human Leucocytes Response to Viable, Extended Freeze-Drying or Heat-Killed Mycobacterium bovis bacillus Calmette-Guerin

被引：5

作者：

Moreira, J. ^{[1
]}

Aragao-Filho, W. C. ^{[1
]}

Barillas, S. G. ^{[2
]}

Barbosa, S. M. ^{[3
]}

Pedroza, L. A. ^{[1
]}

Condino-Neto, A. ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Immunol, Inst Biomed Sci, BR-05508000 Sao Paulo, Brazil

[2] Univ Estadual Campinas, Dept Pharmacol, Sch Med, Campinas, SP, Brazil

[3] Univ Estadual Campinas, Ctr Invest Pediat, Sch Med, Campinas, SP, Brazil

来源：

SCANDINAVIAN JOURNAL OF IMMUNOLOGY | 2012年 / 75卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

TOLL-LIKE RECEPTORS; NADPH OXIDASE ACTIVITY; CLASS-II MOLECULES; NF-KAPPA-B; DENDRITIC CELLS; REACTIVE OXYGEN; IN-VITRO; HUMAN NEUTROPHILS; IMMUNE-RESPONSES; CATHEPSIN-S;

D O I：

10.1111/j.1365-3083.2011.02632.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We investigated the effects of viable, extended freeze-drying (EFD) or heat-killed (HK) Mycobacterium bovis bacillus CalmetteGuerin (BCG) in respiratory burst activity, gene expression of CYBB and NCF1 encoding components of the human phagocyte nicotinamide adenine dinucleotide (NADPH) oxidase, TLR2 expression, and in IL-10 and TNF-a cytokine production by human peripheral blood mononuclear cells (PBMCs). Viable BCG significantly inhibited TLR2 and CYBB gene expression, as well as superoxide release by human PBMC. All BCG stimuli augmented IL-10 release, but only HK BCG or viable BCG increased TNF-a release by PBMCs. Our studies show that viable BCG can impair the NADPH oxidase system activation and the TLR2 route in human PBMCs. As well, different BCG preparations can distinctly influence cytokine production by human PBMCs.

引用

页码：96 / 101

页数：6

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