Main pathways of action of Brazilian red propolis on the modulation of neutrophils migration in the inflammatory process

被引：42
作者：
Bueno-Silva, Bruno [1 ,2 ]
Franchin, Marcelo [2 ]
Alves, Claudiney de Freitas [3 ]
Denny, Carina [2 ]
Colon, David Fernando [4 ]
Cunha, Thiago Mattar [4 ]
Alencar, Severino Matias [5 ]
Napimoga, Marcelo Henrique [6 ]
Rosalen, Pedro Luiz [2 ]
机构：
[1] Univ Guarulhos, Dent Res Div, Guarulhos, SP, Brazil
[2] Univ Estadual Campinas, Sch Dent Piracicaba, Dept Physiol Sci, 901 Limeira Ave, BR-13414903 Piracicaba, SP, Brazil
[3] Univ Fed Uberlandia, Inst Genet & Biochem, Lab Nanobiotechnol & Mol Biol, Uberlandia, MG, Brazil
[4] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Pharmacol, Ribeirao Preto, SP, Brazil
[5] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agrifood Ind Food & Nutr, Piracicaba, SP, Brazil
[6] Sao Leopoldo Mandic Inst & Res Ctr, Lab Immunol & Mol Biol, Campinas, SP, Brazil
来源：
PHYTOMEDICINE | 2016年 / 23卷 / 13期
基金：
巴西圣保罗研究基金会;
关键词：
Brazilian red propolis; Neutrophils; Cytokines; Inflammation; CHEMICAL-COMPOSITION; NITRIC-OXIDE; TNF-ALPHA; VESTITOL; NEOVESTITOL; MECHANISMS; EXTRACT; CELLS; FOCUS;
D O I：
10.1016/j.phymed.2016.09.009
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Background: Brazilian propolis is popularly used as treatment for different diseases including the ones with inflammatory origin. Brazilian red propolis chemical profile and its anti-inflammatory properties were recently described however, its mechanism of action has not been investigated yet. Aim: Elucidate Brazilian red propolis major pathways of action on the modulation of neutrophil migration during the inflammatory process. Methods: The ethanolic extract of propolis (EEP) activity was investigated for neutrophil migration into the peritoneal cavity, intravital microscopy (rolling and adhesion of leukocytes), quantification of cytokines TNF-alpha, IL-1 beta and chemokines CXCL1/KC, CXCL2/MIP-2, neutrophil chemotaxis induced by CXCL2/MIP-2, calcium influx and CXCR2 expression on neutrophils. Results: EEP at 10 mg/kg prevented neutrophil migration into peritoneal cavity (p < 0.05), reduced leukocyte rolling and adhesion on the mesenteric microcirculation (p < 0.05) and inhibited the release TNF-alpha, IL-1 beta, CXCL1/KC and CXCL2/MIP-2 (p < 0.05). EEP at 0.01, 0.1 and 1 mu g/ml reduced the CXCL2/MIP-2-induced neutrophils chemotaxis (p < 0.05) without affect cell viability (p > 0.05). EEP at 1 mu g/ml decreased the calcium influx induced by CXCL2/MIP-2 (p < 0.05). On the other hand, none of EEP concentrations tested altered CXCR2 expression by neutrophils (p > 0.05). Conclusion: Brazilian red propolis appears as a promising anti-inflammatory natural product which mechanism seems to be by reducing leukocyte rolling and adhesion; TNF-alpha, IL-1 beta, CXCL1/KC and CXCL2/MIP-2 release; CXCL2/MIP-2-induced chemotaxis and calcium influx. (C) 2016 Elsevier GmbH. All rights reserved.
引用
收藏
页码：1583 / 1590
页数：8
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