First Detection of Shiga Toxin-Producing Escherichia coli in Shellfish and Coastal Environments of Morocco

被引：20

作者：

Bennani, Mohamed ^{[1
]}

Badri, Samira ^{[1
]}

Baibai, Tarik ^{[2
]}

Oubrim, Nadia ^{[1
]}

Hassar, Mohammed ^{[1
]}

Cohen, Nozha ^{[1
]}

Amarouch, Hamid ^{[3
]}

机构：

[1] Inst Pasteur Maroc, Lab Microbiol & Hyg Aliments & Environm, Casablanca, Morocco

[2] Fac Sci Ain Chok Casablanca, Dept Biol, Lab Physiol & Genet Mol, Casablanca, Morocco

[3] Univ Hassan II Morocco, Fac Sci Ain Chok Casablanca, Dept Biol, Microbiol Lab, Casablanca, Morocco

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2011年 / 165卷 / 01期

关键词：

Coastal environment; Escherichia coli; Shiga-like toxin-producing E. coli; STEC; PCR triplex; POLYMERASE-CHAIN-REACTION; HEMOLYTIC-UREMIC SYNDROME; HEALTHY DOMESTIC-ANIMALS; MULTIPLEX PCR; FOODBORNE DISEASES; CASABLANCA MOROCCO; VIRULENCE FACTORS; GENETIC-LOCUS; FECAL SAMPLES; EAE GENE;

D O I：

10.1007/s12010-011-9251-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Shiga toxin Escherichia coli (STEC), also called verotoxin-producing E. coli, is a major cause of food-borne illness, capable of causing hemorrhagic colitis and hemolytic-uremic syndrome (HUS). This study was carried out to evaluate the presence of (STEC) and E. coli O157:H7 in shellfish and Mediterranean coastal environments of Morocco. The contamination of shellfish and marine environment with Shiga toxin-producing E. coli (STEC) and E. coli O157:H7, was investigated during 2007 and 2008. A total of 619 samples were analyzed and 151 strains of E. coli were isolated. The presence of the stx1, stx2, and eae genes was tested in E. coli isolates strains using a triplex polymerase chain reaction. STEC was detected in three positives samples (1.9%), corresponding to the serotype O157:H7, the others Shiga toxin-producing E. coli non-O157 were also detected.

引用

页码：290 / 299

页数：10

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