Quantification of propane in biological materials by head-space GC

Propane was measured in specimens taken from two persons who died from a LPG explosion in an apartment using headspace-GC/FID. Because of the variation of instrument performance and sample injection, the internal standard pentane was used. Calibration standards were prepared by injecting each calculated volume of pure propane gas into capped vials containing 2 ml of blood and 5 mu l of internal standard. The calibration curve revealed good linearity from 0.09 mu g/ml to at least 90 mu g/ml. The method validation data also included repeatability and recovery. The propane quantities in blood, fat and brain tissue were between 0.27 and 71 mu g/ml (mu g/g) with the highest concentration observed in fat. The confirmation of propane was conducted by the means of solid phase micro-extraction and mass spectrometry. (c) 2005 Elsevier Ireland Ltd. All rights reserved.