Silicon-based microfilters for whole blood cell separation

被引：225

作者：

Ji, Hong Miao ^{[1
]}

Samper, Victor ^{[1
]}

Chen, Yu ^{[1
]}

Heng, Chew Kiat ^{[2
]}

Lim, Tit Meng ^{[3
]}

Yobas, Levent ^{[1
]}

机构：

[1] Inst Microelect, Singapore, Singapore

[2] Natl Univ Singapore, Dept Pediat, Singapore 117548, Singapore

[3] Natl Univ Singapore, Dept Biol Sci, Singapore 117548, Singapore

来源：

BIOMEDICAL MICRODEVICES | 2008年 / 10卷 / 02期

关键词：

microfluidic; filter; cross-flow; silicon; separation; lab on a chip;

D O I：

10.1007/s10544-007-9131-x

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

This paper reports on the comparison analysis of four main types of silicon-based microfilter for isolation of white blood cells (WBCs) from red blood cells (RBCs) in a given whole blood. The microfilter designs, namely, weir, pillar, crossflow, and membrane, all impose the same cut-off size of 3.5 mu m to selectively trap WBCs. Using human whole blood, the microfilters have been characterized and compared for their blood handling capacity, WBCs trapping efficiency and RBCs passing efficiency. Based on the experimental results, the crossflow microfilter is superior and can be integrated with downstream components for on-chip genomic analysis.

引用

收藏

页码：251 / 257

页数：7

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