The variability of nuclear DNA content of different Pelargonium species estimated by flow cytometry

被引:7
作者
Plaschil, Sylvia [1 ]
Abel, Simone [1 ]
Klocke, Evelyn [1 ]
机构
[1] Julius Kuhn Inst JKI, Inst Breeding Res Hort Crops, Fed Res Ctr Cultivated Plant, Quedlinburg, Germany
来源
PLOS ONE | 2022年 / 17卷 / 04期
关键词
SECTION LIGULARIA; GENOME SIZE; GERANIACEAE; MITOCHONDRIAL; PHYLOGENY; EVOLUTION; CAPE; REAPPRAISAL; ENDOPOLYPLOIDY; REVISION;
D O I
10.1371/journal.pone.0267496
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Pelargonium is a versatile genus mainly from the Cape Region, South Africa. The genus is divided into four subgenera and 16 sections characterized by several groups of chromosomes sizes and numbers. The DNA content of species from all subgenera and sections of Pelargonium, except for the sections Subsucculentia and Campylia was estimated using flow cytometry. Nuclei of Pelargonium samples (leaf or petal tissue) and an internal plant standard (leaf tissue) were isolated together and stained with propidium iodide. The DNA content was estimated providing that the 2C peaks of sample and standard be in linearity in the flow cytometer histograms. In total, 96 Pelargonium accessions of 60 species (22 Pelargonium species for the first time) were analyzed. The 2C DNA content ranged from 0.84 pg (P. longifolium, section Hoarea) to 6.69 pg (P. schizopetalum, section Magnistipulacea) and the corresponding 1Cx DNA content from 0.42 pg (P. longifolium) to 1.72 pg (P. transvaalense. This demonstrates the high plasticity within the genus Pelargonium. Some species, such as P. peltatum accessions revealed a pronounced endopolyploidization in leaves but not in petals underlining the importance to choose the right tissue as sample for the flow cytometry analysis. The reported genome sizes are a step forward towards the characterization of the Pelargonium collection within the German Gene Bank for Ornamental Plants and a valuable base for future sequencing programs of the Pelargonium genomes.
引用
收藏
页数:17
相关论文
共 61 条
  • [1] A biosystematic study of Pelargonium section Ligularia .2. Reappraisal of section Chorisma
    Albers, F
    vanderWalt, JJA
    Gibby, M
    Marschewski, DE
    Price, RA
    duPreez, G
    [J]. SOUTH AFRICAN JOURNAL OF BOTANY, 1995, 61 (06) : 339 - 346
  • [2] Biosystematic study of Pelargonium section Ligularia:: 4.: The section Ligularia sensu stricto
    Albers, F
    van der Walt, JJA
    Gibby, M
    Marschewski, DE
    van der Merwe, AM
    Marais, EM
    Bakker, FT
    Culham, A
    [J]. SOUTH AFRICAN JOURNAL OF BOTANY, 2000, 66 (01) : 31 - 43
  • [3] A REAPPRAISAL OF PELARGONIUM SECT LIGULARIA (GERANIACEAE)
    ALBERS, F
    GIBBY, M
    AUSTMANN, M
    [J]. PLANT SYSTEMATICS AND EVOLUTION, 1992, 179 (3-4) : 257 - 276
  • [4] STUDIES IN KARYOLOGY AND MICROSPOROGENESIS OF PELARGONIUM SECT PELARGONIUM (GERANIACEAE)
    ALBERS, F
    VANDERWALT, JJA
    [J]. PLANT SYSTEMATICS AND EVOLUTION, 1984, 147 (3-4) : 177 - 188
  • [5] [Anonymous], 2004, SCHUMANNIA
  • [6] [Anonymous], [No title captured]
  • [7] Biparental inheritance of organelles in Pelargonium: evidence for intergenomic recombination of mitochondrial DNA
    Apitz, Janina
    Weihe, Andreas
    Pohlheim, Frank
    Boerner, Thomas
    [J]. PLANTA, 2013, 237 (02) : 509 - 515
  • [8] DNA sequence evolution in fast evolving mitochondrial DNA nad1 exons in Geraniaceae and Plantaginaceae
    Bakker, Freek T.
    Breman, Floris
    Merckx, Vincent
    [J]. TAXON, 2006, 55 (04) : 887 - 896
  • [9] Mitochondrial and chloroplast DNA-based phylogeny of Pelarogonium (Geraniaceae)
    Bakker, FT
    Culham, A
    Pankhurst, CE
    Gibby, M
    [J]. AMERICAN JOURNAL OF BOTANY, 2000, 87 (05) : 727 - 734
  • [10] Bakker FT, 2005, REGNUM VEG, V143, P75