Development and applications of a monoclonal antibody to a recombinant beak and feather disease virus (BFDV) capsid protein

被引：11

作者：

Shearer, Patrick L. ^{[2
,3
]}

Bonne, Nicolai ^{[2
,3
]}

Clark, Phillip ^{[2
]}

Sharp, Margaret ^{[2
]}

Raidal, Shane R. ^{[1
]}

机构：

[1] Charles Sturt Univ, Sch Agr & Vet Sci, Wagga Wagga, NSW 2650, Australia

[2] Murdoch Univ, Sch Vet & Biomed Sci, Perth, WA 6150, Australia

[3] Murdoch Univ, State Agr Biotechnol Ctr, Perth, WA 6150, Australia

来源：

JOURNAL OF VIROLOGICAL METHODS | 2008年 / 147卷 / 02期

关键词：

monoclonal antibody; beak and feather disease virus; circovirus; immunohistochemistry; haemagglutination-inhibition;

D O I：

10.1016/j.jviromet.2007.08.029

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of diagnostic assays for detecting beak and feather disease virus (BFDV) has traditionally been hampered by the difficulty associated with producing suitable reagents, namely purified virus and polyclonal antibodies. In an effort to develop a consistent and standardised source of antibody, a monoclonal antibody to a recombinant BFDV capsid protein has been developed and its use in western blotting, immunohistochemistry (IHC), ELISA and haemagglutination-inhibition (HI) assays characterised. The antibody was specific for both the recombinant BFDV capsid protein and the whole virus and had similar optimal titres when used in western blotting and IHC. The antibody also had HI activity and detected BFDV virus from three genera of psittacine birds, including the recently described cockatiel BFDV isolate. The monoclonal antibody should have widespread application in both research and the development of diagnostic assays for BFDV. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：206 / 212

页数：7

共 25 条

[1]

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[2] Genetic diversity of beak and feather disease virus detected in psittacine species in Australia [J].