The Rhodobacter capsulatus gene transfer agent is induced by nutrient depletion and the RNAP omega subunit

被引：23

作者：

Westbye, Alexander B. ^{[1
,2
]}

O'Neill, Zoe ^{[1
]}

Schellenberg-Beaver, Tegan ^{[1
]}

Beatty, J. Thomas ^{[1
]}

机构：

[1] Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC, Canada

[2] NIOZ Royal Netherlands Inst Sea Res, Marine Microbiol & Biogeochem, T Horntje, Texel, Netherlands

来源：

MICROBIOLOGY-SGM | 2017年 / 163卷 / 09期

基金：

加拿大健康研究院;

关键词：

Rhodobacter capsulatus; GTA; spoT; rpoZ; RNAP omega subunit; nutrient depletion; ppGpp; QUORUM-SENSING REGULATION; RECIPIENT CAPABILITY; RHODOPSEUDOMONAS-CAPSULATA; PSEUDOMONAS-STUTZERI; PPGPP BINDING; POLYMERASE; PROTEIN; SEQUENCE; TRANSCRIPTION; TRANSFORMATION;

D O I：

10.1099/mic.0.000519

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Small bacteriophage-like particles called gene transfer agents (GTAs) that mediate DNA transfer between cells are produced by a variety of prokaryotes. The model GTA, produced by the alphaproteobacterium Rhodobacter capsulatus (RcGTA), is controlled by several cellular regulators, and production is induced upon entry into the stationary phase. We report that RcGTA production and gene transfer are stimulated by nutrient depletion. Cells depleted of organic carbon or blocked for amino acid biosynthesis increased RcGTA production and release from cells. Furthermore, cells lacking the sole RelA-SpoT homologue produced decreased levels of RcGTA, and the RNA polymerase omega (omega) subunit was required for appreciable production of RcGTA.

引用

页码：1355 / 1363

页数：9

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