SARS-CoV-2 recombinant proteins stimulate distinct cellular and humoral immune response profiles in samples from COVID-19 convalescent patients

被引：8

作者：

da Silva, Lais Teodoro ^{[1
]}

Ortega, Marina Mazzilli ^{[1
]}

Tiyo, Bruna Tiaki ^{[1
]}

Tabosa Viana, Isabelle Freire ^{[3
]}

de Lima, Tayna Evily ^{[3
]}

Tozetto-Mendoza, Tania Regina ^{[2
]}

da Silva Oliveira, Luanda Mara ^{[1
]}

Emidio Teixeira, Franciane Mouradian ^{[1
]}

Lins, Roberto Dias ^{[3
]}

de Almeida, Alexandre ^{[1
]}

Mendes-Correa, Maria Cassia ^{[2
]}

da Silva Duarte, Alberto Jose ^{[1
,4
]}

Oshiro, Telma Miyuki ^{[1
]}

机构：

[1] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Lab Invest Med Dermatol & Imunodeficiencias LIM 5, Sao Paulo, SP, Brazil

[2] Univ Sao Paulo, Hosp Clin HCFMUSP, Fac Med, Lab Virol LIM 52, Sao Paulo, SP, Brazil

[3] Fundacao Oswaldo Cruz, Inst Aggeu Magalhaes, Dept Virol, Recife, PE, Brazil

[4] Univ Sao Paulo, Hosp Clin HCFMUSP, Fac Med, Div Lab Cent, Sao Paulo, SP, Brazil

来源：

CLINICS | 2021年 / 76卷

基金：

巴西圣保罗研究基金会;

关键词：

SARS-CoV-2; COVID-19; Antigens; Immune Response; CD4(+);

D O I：

10.6061/clinics/2021/e3548

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

OBJECTIVES: In this preliminary study we investigated cellular and humoral immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens in blood samples from 14 recovered coronavirus disease 2019 (COVID-19) patients and compared them to those in samples from 12 uninfected/unvaccinated volunteers. METHODS: Cellular immunity was assessed by intracellular detection of IFN-g in CD3+ T lymphocytes after stimulation with SARS-CoV-2 spike (S1), nucleocapsid (NC), or receptor-binding domain (RBD) recombinant proteins or overlapping peptide pools covering the sequence of SARS-CoV-2 spike, membrane and nucleocapsid regions. The humoral response was examined by ELISAs and/or chemiluminescence assays for the presence of serum IgG antibodies directed to SARS-CoV-2 proteins. RESULTS: We observed differences between humoral and cellular immune profiles in response to stimulation with the same proteins. Assays of IgG antibodies directed to SARS-CoV-2 NC, RBD and S1/S2 recombinant proteins were able to differentiate convalescent from uninfected/unvaccinated groups. Cellular immune responses to SARS-CoV-2 protein stimuli did not exhibit a specific response, as T cells from both individuals with no history of contact with SARS-CoV-2 and from recovered donors were able to produce IFN-g. CONCLUSIONS: Determination of the cellular immune response to stimulation with a pool of SARS-CoV-2 peptides but not with SARS-CoV-2 proteins is able to distinguish convalescent individuals from unexposed individuals. Regarding the humoral immune response, the screening for serum IgG antibodies directed to SARSCoV-2 proteins has been shown to be specific for the response of recovered individuals.

引用

页数：11

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