Gamma radiation effects on alpha-lactalbumin:: structural modifications

被引：8
作者：
Chapelier, A
Desmadril, M
Houée-Levin, C
机构：
[1] Univ Paris Sud, CNRS, UMR 8611, Chim Phys Lab, F-91405 Orsay, France
[2] Univ Paris Sud, Lab MIP, F-91405 Orsay, France
来源：
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | 2001年 / 79卷 / 02期
关键词：
ionizing radiation; radiation-induced conformational changes; protein structural modifications; protein thermodynamic properties;
D O I：
10.1139/cjpp-79-2-154
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
alpha -Lactalbumin was irradiated in the lyophilized state in air at ambient temperature. The irradiated protein was examined by size exclusion chromatography, sodium dodecyl sulfate polyacrylamide gel electrophoresis, circular dichroism, and microcalorimetry. Irradiation induced the loss of aromatic amino acids and of helicity so that fragmentation and aggregation products were obtained. The thermodynamic properties of the protein were also modified. The irradiated protein had lower stability, however, the temperature at which denaturation occurred process remained constant.
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页码：154 / 157
页数：4
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