Hemin activation abrogates Mycoplasma hyorhinis replication in chronically infected prostate cancer cells via heme oxygenase-1 induction

被引：3

作者：

Huang, Hanxia ^{[1
]}

Dabrazhynetskaya, Alena ^{[1
]}

Pluznik, Jacob ^{[1
]}

Zheng, Jiwen ^{[2
]}

Wu, Yong ^{[2
]}

Chizhikov, Vladimir ^{[1
]}

Buehler, Paul W. ^{[1
,4
]}

Yamada, Kenneth M. ^{[3
]}

Dhawan, Subhash ^{[1
]}

机构：

[1] US FDA, Ctr Biol Evaluat & Res, Silver Spring, MD USA

[2] US FDA, Off Sci & Engn Labs, Ctr Devices & Radiol Hlth, Silver Spring, MD USA

[3] Natl Inst Dent & Craniofacial Res, NIH, Bethesda, MD USA

[4] Univ Maryland, Sch Med, Ctr Blood Oxygen Transport & Hemostasis, Dept Pathol,Dept Pediat, Baltimore, MD 21201 USA

来源：

FEBS OPEN BIO | 2021年 / 11卷 / 10期

关键词：

heme oxygenase-1; host defense; infectious disease; innate immunity; Mycoplasma hyorhinis; prostate cancer; EXPRESSION; P37; MORPHOLOGY; NRF2;

D O I：

10.1002/2211-5463.13271

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mycoplasma hyorhinis (M. hyorhinis) lacks a cell wall and resists multiple antibiotics. We describe here the striking > 90% inhibitory effect of hemin, a natural inducer of the cytoprotective enzyme heme oxygenase-1 (HO-1), on M. hyorhinis replication in chronically infected LNCaP prostate cancer cells. The role of HO-1 in interrupting M. hyorhinis replication was confirmed by HO-1-specific siRNA suppression of hemin-induced HO-1 protein expression, which increased intracellular M. hyorhinis DNA levels in LNCaP cells. Proteomic analysis and transmission electron microscopy of hemin-treated cells confirmed the complete absence of M. hyorhinis proteins and intact microorganisms, respectively, strongly supporting these findings. Our study is the first to our knowledge suggesting therapeutic potential for activated HO-1 in cellular innate responses against mycoplasma infection.

引用

页码：2727 / 2739

页数：13

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