Diltiazem potentiation of doxorubicin cytotoxicity and cellular uptake in human breast cancer cells

被引：4
作者：
Al-malky, Hamdan S. [1 ]
Damanhouri, Zoheir A. [1 ]
Al Aama, Jumana Y. [2 ]
Al Qahtani, Ali A. [1 ]
Ramadan, Wafaa S. [1 ,3 ]
AlKreathy, Huda M. [1 ]
Al Harthi, Sameer E. [1 ]
Osman, Abdel-Moneim M. [1 ,4 ]
机构：
[1] King Abdulaziz Univ, Fac Med, Dept Pharmacol, Jeddah, Saudi Arabia
[2] King Abdulaziz Univ, Fac Med, Dept Genet, Jeddah, Saudi Arabia
[3] Ain Shams Univ, Fac Med, Dept Anat, Cairo, Egypt
[4] Cairo Univ, Pharmacol Unit, NCI, Cairo, Egypt
来源：
BREAST CANCER MANAGEMENT | 2019年 / 8卷 / 04期
关键词：
activity enhancement; diltiazem; doxorubicin; MCF-7; cells; multidrug resistance; P-GLYCOPROTEIN; INHIBITION; RESISTANCE; DOCETAXEL; EXPRESSION; MECHANISM; APOPTOSIS; EFFICACY; GROWTH; DEATH;
D O I：
10.2217/bmt-2019-0018
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Aim: Breast cancer is the most common cancer among Arab women and also around the world. Chronic cardiotoxicity and multidrug resistance are potential limiting factors of doxorubicin (DOX), a known anthracycline antibiotic. Materials & methods: DOX cytotoxicity was evaluated by the sulforhodamine method. DOX cellular uptake, detection of P-glycoprotein activity and the photomicrograph of MCF-7 cells were also determined. Results: Diltiazem (DIL) treatment improved DOX cytotoxic activity and increased the cellular uptake of DOX significantly and aggregation of rhodamine 123, reflecting inhibition of P-glycoprotein pump. Cytopathological investigation of MCF-7 cells revealed marked cytotoxic activity of DOX in the presence of DIL. Conclusion: DIL treatment enhanced DOX cytotoxic effect and reduced multidrug resistance, which increased the drug accumulation intracellularly.
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页数：10
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