Methylation level of the RASSF1A promoter is an independent prognostic factor for clear-cell renal cell carcinoma

被引：41

作者：

Kawai, Y. ^{[1
]}

Sakano, S. ^{[1
]}

Suehiro, Y. ^{[2
]}

Okada, T. ^{[2
]}

Korenaga, Y. ^{[1
]}

Hara, T. ^{[1
]}

Naito, K. ^{[1
]}

Matsuyama, H. ^{[1
]}

Hinoda, Y. ^{[2
]}

机构：

[1] Yamaguchi Univ, Grad Sch Med, Dept Urol, Yamaguchi 7558505, Japan

[2] Yamaguchi Univ, Grad Sch Med, Dept Oncol & Lab Med, Yamaguchi 7558505, Japan

来源：

ANNALS OF ONCOLOGY | 2010年 / 21卷 / 08期

基金：

日本学术振兴会;

关键词：

COBRA; kidney cancer; survival; tumor suppressor gene; TUMOR-SUPPRESSOR GENE; FREQUENT EPIGENETIC INACTIVATION; ASSOCIATION DOMAIN FAMILY-1; DNA METHYLATION; LUNG-CANCER; HYPERMETHYLATION; 3P21.3; CLASSIFICATION; IDENTIFICATION; TUMORIGENESIS;

D O I：

10.1093/annonc/mdp577

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Ras association domain family 1A (RASSF1A) is a tumor suppressor that regulates the cell cycle, apoptosis, and microtubule stability. The association between the methylation levels of RASSF1A and the prognosis of clear-cell renal cell carcinoma (CCRCC) remains unclear. Therefore, we investigated this relationship to determine the prognostic value of RASSF1A methylation levels for CCRCC. Patients and methods: The study comprised 179 Japanese patients who underwent radical or partial nephrectomy for CCRCC. The methylation level of 5' CpG islands in the RASSF1A was evaluated using combined bisulfite restriction analysis and bisulfite sequencing. Results: High levels of methylation in the RASSF1A promoter were significantly more frequent in grade 3 compared with grade 1 or 2 tumors (P = 0.028) and in patients with stage III or IV compared with patients with stage I or II (P = 0.043). Patients with high methylation levels had a significantly less favorable prognosis compared with those with low methylation levels (P = 0.040). Higher methylation levels were independently associated with a poor prognosis following multivariate analysis (P = 0.0053). Conclusion: These results indicate that quantitative promoter methylation levels of the RASSF1A gene may be a useful marker to predict the prognosis of CCRCC.

引用

页码：1612 / 1617

页数：6

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