Improving Consistency of Photobiomodulation Therapy: A Novel Flat-Top Beam Hand-Piece versus Standard Gaussian Probes on Mitochondrial Activity

被引:25
作者
Amaroli, Andrea [1 ,2 ]
Arany, Praveen [3 ,4 ,5 ]
Pasquale, Claudio [2 ]
Benedicenti, Stefano [2 ]
Bosco, Alessandro [6 ]
Ravera, Silvia [7 ]
机构
[1] First Moscow State Med Univ, Fac Dent, Dept Orthoped Dent, Sechenov Univ, Moscow 119991, Russia
[2] Univ Genoa, Dept Surg & Diagnost Sci, I-16132 Genoa, Italy
[3] Univ Buffalo, Dept Oral Biol, Buffalo, NY 14260 USA
[4] Univ Buffalo, Dept Surg, Buffalo, NY 14260 USA
[5] Univ Buffalo, Dept Biomed Engn, Buffalo, NY 14260 USA
[6] Bosco Ott Srl, I-24040 Bergamo, Italy
[7] Univ Genoa, Dept Expt Med, I-16132 Genoa, Italy
关键词
phototherapy; light therapy; low-level laser therapy; ATP; mitochondria respiratory chain; light-emitting diode; near-infrared light; energetic metabolism; LASER; COLOR; IR;
D O I
10.3390/ijms22157788
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The tremendous therapeutic potential of photobiomodulation therapy in different branches of medicine has been described in the literature. One of the molecular mechanisms for this treatment implicates the mitochondrial enzyme, cytochrome C oxidase. However, the efficacy and consistency of clinical outcomes with photobiomodulation treatments has been fiercely debated. This work was motivated by this need to improve photobiomodulation devices and delivery approaches. We designed a novel hand-piece with a flat-top beam profile of irradiation. We compared the beam profile versus a standard hand-piece and a fibre probe. We utilized isolated mitochondria and performed treatments at various spots within the beam, namely, the centre, left and right edge. We examined mitochondrial activity by assessing ATP synthesis with the luciferin/luciferase chemiluminescent method as a primary endpoint, while mitochondrial damage was assessed as the secondary endpoint. We observed a uniform distribution of the power density with the flat-top prototype compared to a wide Gaussian beam profile with the standard fibre and standard hand-piece. We noted increased production of ATP in the centre of all three beams with respect to the non-treated controls (p < 0.05). Both the fibre and standard hand-piece demonstrated less increase in ATP synthesis at the edges than the centre (p < 0.05). In contrast, ATP synthesis was increased homogenously in the flat-top handpiece, both in the centre and the edges of the beam. Fibre, standard hand-piece and the flat-top hand-piece prototype have discrete beam distribution characteristics. This significantly affected the mitochondrial activity with respect to their position within the treated areas. Flat-top hand-piece enhances the uniformity of photobiomodulation treatments and can improve the rigour and reproducibility of PBM clinical outcomes.
引用
收藏
页数:10
相关论文
共 27 条
[1]  
Amaroli A., 2021, BIOELECTROMAGNETICS, V18, P22342
[2]   Photobiomodulation and Oxidative Stress: 980 nm Diode Laser Light Regulates Mitochondrial Activity and Reactive Oxygen Species Production [J].
Amaroli, Andrea ;
Pasquale, Claudio ;
Zekiy, Angelina ;
Utyuzh, Anatoliy ;
Benedicenti, Stefano ;
Signore, Antonio ;
Ravera, Silvia .
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2021, 2021
[3]   Interaction between Laser Light and Osteoblasts: Photobiomodulation as a Trend in the Management of Socket Bone Preservation-A Review [J].
Amaroli, Andrea ;
Colombo, Esteban ;
Zekiy, Angelina ;
Aicardi, Stefano ;
Benedicenti, Stefano ;
De Angelis, Nicola .
BIOLOGY-BASEL, 2020, 9 (11) :1-15
[4]   Photobiomodulation Affects Key Cellular Pathways of all Life-Forms: Considerations on Old and New Laser Light Targets and the Calcium Issue [J].
Amaroli, Andrea ;
Ferrando, Sara ;
Benedicenti, Stefano .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 2019, 95 (01) :455-459
[5]   An 808-nm Diode Laser with a Flat-Top Handpiece Positively Photobiomodulates Mitochondria Activities [J].
Amaroli, Andrea ;
Ravera, Silvia ;
Parker, Steven ;
Panfoli, Isabella ;
Benedicenti, Alberico ;
Benedicenti, Stefano .
PHOTOMEDICINE AND LASER SURGERY, 2016, 34 (11) :564-571
[6]   Craniofacial Wound Healing with Photobiomodulation Therapy: New Insights and Current Challenges [J].
Arany, P. R. .
JOURNAL OF DENTAL RESEARCH, 2016, 95 (09) :977-984
[7]   Low-Level Laser Therapy Activates NF-kB via Generation of Reactive Oxygen Species in Mouse Embryonic Fibroblasts [J].
Chen, Aaron C-H ;
Arany, Praveen R. ;
Huang, Ying-Ying ;
Tomkinson, Elizabeth M. ;
Sharma, Sulbha K. ;
Kharkwal, Gitika B. ;
Saleem, Taimur ;
Mooney, David ;
Yull, Fiona E. ;
Blackwell, Timothy S. ;
Hamblin, Michael R. .
PLOS ONE, 2011, 6 (07)
[8]   Experimental and Clinical Applications of Red and Near-Infrared Photobiomodulation on Endothelial Dysfunction: A Review [J].
Colombo, Esteban ;
Signore, Antonio ;
Aicardi, Stefano ;
Zekiy, Angelina ;
Utyuzh, Anatoliy ;
Benedicenti, Stefano ;
Amaroli, Andrea .
BIOMEDICINES, 2021, 9 (03)
[9]   Photobiomodulation in Pediatric Dentistry: A Current State-of-the-Art [J].
Fornaini, Carlo ;
Arany, Praveen ;
Rocca, Jean-Paul ;
Merigo, Elisabetta .
PHOTOBIOMODULATION PHOTOMEDICINE AND LASER SURGERY, 2019, 37 (12) :798-813
[10]   A Comparative Study Between the Effectiveness of 980 nm Photobiomodulation Delivered by Hand-Piece With Gaussian vs. Flat-Top Profiles on Osteoblasts Maturation [J].
Hanna, Reem ;
Agas, Dimitrios ;
Benedicenti, Stefano ;
Ferrando, Sara ;
Laus, Fulvio ;
Cuteri, Vincenzo ;
Lacava, Giovanna ;
Sabbieti, Maria Giovanna ;
Amaroli, Andrea .
FRONTIERS IN ENDOCRINOLOGY, 2019, 10