Nerve growth factor-stimulated nuclear S6 kinase in PC12 cells

被引：0

作者：

Tang, T ^{[1
]}

Hirata, Y ^{[1
]}

Whalin, M ^{[1
]}

Guroff, G ^{[1
]}

机构：

[1] NICHHD, NIH, GROWTH FACTORS SECT, BETHESDA, MD 20892 USA

来源：

JOURNAL OF NEUROCHEMISTRY | 1996年 / 66卷 / 03期

关键词：

PC12; cells; nerve growth factor; nuclear S6 kinase;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous work has shown that nerve growth factor (NGF) stimulates the phosphorylation of the ribosomal protein S6 in PC12 cells. In this study, we show that S6 kinase activity is also present in purified PC12 cell nuclei. This activity was increased by treatment of the cells with NGF and, to a lesser extent, by treatment with epidermal growth factor. The NGF-stimulated activity was obtained from nuclear extracts and some of its characteristics described. The increase in activity was prevented by treatment of the cells with rapamycin or with wortmannin, and the overall activity could be precipitated by antibodies directed against the p85(S6K). These data indicate that p85(S6K) is the NGF-stimulated S6 kinase in PC12 cell nuclei. The presence of S6 protein in the nucleus of PC12 cells has been confirmed and evidence is presented that suggests that it is identical to a protein called SMP reported some years ago.

引用

页码：1198 / 1206

页数：9

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