Differential Gene Expression in Ovaries of Qira Black Sheep and Hetian Sheep Using RNA-Seq Technique

被引：67

作者：

Chen, Han Ying ^{[1
]}

Shen, Hong ^{[2
]}

Bin Jia ^{[2
]}

Zhang, Yong Sheng ^{[2
]}

Wang, Xu Hai ^{[2
]}

Zeng, Xian Cun ^{[2
]}

机构：

[1] Shihezi Univ, Sch Pharm, Shihezi, Xinjiang, Peoples R China

[2] Shihezi Univ, Coll Anim Sci & Technol, Shihezi, Xinjiang, Peoples R China

来源：

PLOS ONE | 2015年 / 10卷 / 03期

基金：

中国国家自然科学基金;

关键词：

ENDOTHELIAL GROWTH-FACTOR; FOLLICULAR DEVELOPMENT; GRANULOSA-CELLS; MESSENGER-RNA; FACTOR VEGF; FACTOR-BETA; IN-VITRO; TRANSCRIPTOME; IDENTIFICATION; FOLLICLES;

D O I：

10.1371/journal.pone.0120170

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Qira black sheep and the Hetian sheep are two local breeds in the Northwest of China, which are characterized by high-fecundity and low-fecundity breed respectively. The elucidation of mRNA expression profiles in the ovaries among different sheep breeds representing fecundity extremes will helpful for identification and utilization of major prolificacy genes in sheep. In the present study, we performed RNA-seq technology to compare the difference in ovarian mRNA expression profiles between Qira black sheep and Hetian sheep. From the Qira black sheep and the Hetian sheep libraries, we obtained a total of 11,747,582 and 11,879,968 sequencing reads, respectively. After aligning to the reference sequences, the two libraries included 16,763 and 16,814 genes respectively. A total of 1,252 genes were significantly differentially expressed at Hetian sheep compared with Qira black sheep. Eight differentially expressed genes were randomly selected for validation by real-time RTPCR. This study provides a basic data for future research of the sheep reproduction.

引用

页数：15

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