Polyhemoglobin-superoxide Dismutase-catalase-carbonic Anhydrase: A Novel Biotechnology-based Blood Substitute that Transports both Oxygen and Carbon Dioxide and also Acts as an Antioxidant

被引：41

作者：

Bian, Yuzhu

Rong, Zhixia

Chang, Thomas Ming Swi ^{[1
]}

机构：

[1] McGill Univ, Fac Med, Artificial Cells & Organs Res Ctr, Dept Physiol, Montreal, PQ H3G 1Y6, Canada

来源：

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2011年 / 39卷 / 03期

基金：

加拿大健康研究院;

关键词：

HEMOGLOBIN; REPLACEMENT;

D O I：

10.3109/10731199.2011.581052

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase (PolyHb-SOD-CAT-CA) is a therapeutic antioxidant that also transports both oxygen and carbon dioxide. This is formed by crosslinking Hb with SOD, CAT, and CA using glutaraldehyde. Crosslinking stroma free Hb from red blood cell (rbc) reduces CA activity to 55%. Addition of more CA resulted in a preparation with the same CA activity as RBC. PolyHb in the complex acts as a buffer to prevent large pH changes as carbon dioxide is converted to carbonic acid. We then prepare and optimize a novel PolyHb-SOD-CAT-CA, a therapeutic antioxidant that also transports both oxygen and carbon dioxide.

引用

页码：127 / 136

页数：10

共 21 条

[1] Carbon dioxide transport [J].