Molecular characterization of early adenocarcinoma of the uterine cervix by oligonucleotide microarray

被引:1
作者
Liao, Ling-Min [2 ,3 ]
Zheng, Min [1 ,4 ]
Huang, Long [1 ,4 ]
Ding, Hui [1 ,4 ]
He, Li [1 ,4 ]
Zhang, Ying [5 ]
Wang, Hui-Yun [4 ]
机构
[1] Sun Yat Sen Univ, Dept Gynecol, Ctr Canc, Guangzhou 510060, Guangdong, Peoples R China
[2] Shantou Univ, Dept Radiol, Affiliated Hosp 1, Coll Med, Shantou 515041, Peoples R China
[3] Guangdong Key Lab Med Mol Imaging, Shantou 515041, Peoples R China
[4] State Key Lab Oncol So China, Guangzhou 510060, Guangdong, Peoples R China
[5] Sun Yat Sen Univ, Dept Sample Bank, Ctr Canc, Guangzhou 510060, Guangdong, Peoples R China
关键词
Adenocarcinoma; Uterine cervix; Gene expression; Oligonucleotide microarray; SQUAMOUS-CELL CARCINOMA; GENE-EXPRESSION; BREAST-CANCER; IONIZING-RADIATION; IN-SITU; SURVIVAL; IDENTIFICATION; SENSITIVITY; PROGRESSION; PROGNOSIS;
D O I
10.1007/s00404-010-1511-4
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
In an attempt to understand the molecular characterization for the early adenocarcinoma of the uterine cervix, an analysis of gene expression profiles obtained from early adenocarcinoma of the uterine cervix was performed to find those genes most aberrantly expressed. Total RNA was prepared from 32 samples of early adenocarcinoma of the uterine cervix and 32 paired normal cervix tissues, and hybridized to cancer-associated oligonucleotide microarrays with probe sets complementary to about 1,426 transcripts. Supervised analysis of gene expression data identified 13 genes that exhibited > 2-fold upregulation and 27 genes > 2-fold downregulation, respectively, in early adenocarcinoma of the uterine cervix compared to normal cervix. Unsupervised hierarchical clustering of the expression data readily distinguished early adenocarcinoma of the uterine cervix from normal cervix. Two genes (karyopherin alpha 2 and proliferating cell nuclear antigen) were selected randomly for real-time reverse transcription polymerase chain reaction analysis. Both genes were expressed significantly higher in early adenocarcinoma of the uterine cervix than in normal cervix, with p = 0.0003 and < 0.0001, respectively. These results were compatible with the microarray data. This study has revealed several genes that may be highly attractive candidate molecular markers/targets for early adenocarcinoma of the uterine cervix.
引用
收藏
页码:861 / 869
页数:9
相关论文
共 41 条
[1]   Cyclooxygenase-independent induction of apoptosis by sulindac sulfone is mediated by polyamines in colon cancer [J].
Babbar, N ;
Ignatenko, NA ;
Casero, RA ;
Gerner, EW .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (48) :47762-47775
[2]   Radioresistant cervical cancer shows upregulation of the NHEJ proteins DNA-PKcs, Ku70 and Ku86 [J].
Beskow, C. ;
Skikuniene, J. ;
Holgersson, A. ;
Nilsson, B. ;
Lewensohn, R. ;
Kanter, L. ;
Viktorsson, K. .
BRITISH JOURNAL OF CANCER, 2009, 101 (05) :816-821
[3]   Adhesion molecules and p16 expression in endocervical adenocarcinoma [J].
Carico, Elisabetta ;
Fulciniti, Franco ;
Giovagnoli, Maria Rosaria ;
Losito, Nunzia Simona ;
Botti, Gerardo ;
Benincasa, Giulio ;
Farnetano, Maria Giuseppina ;
Vecchione, Aldo .
VIRCHOWS ARCHIV, 2009, 455 (03) :245-251
[4]   Changes in cervical cancer incidence after three decades of screening US women less than 30 years old [J].
Chan, PG ;
Sung, HY ;
Sawaya, GF .
OBSTETRICS AND GYNECOLOGY, 2003, 102 (04) :765-773
[5]   Molecular characterization of adenocarcinoma and squamous carcinoma of the uterine cervix using microarray analysis of gene expression [J].
Chao, Angel ;
Wang, Tzu-Hao ;
Lee, Yun-Shien ;
Hsueh, Swei ;
Chao, An-Shine ;
Chang, Ting- Chang ;
Kung, Wei-Hsiang ;
Huang, Shang-Lang ;
Chao, Fang-Yu ;
Wei, Min-Li ;
Lai, Chyong-Huey .
INTERNATIONAL JOURNAL OF CANCER, 2006, 119 (01) :91-98
[6]  
Chao Angel, 2007, Taiwan J Obstet Gynecol, V46, P363, DOI 10.1016/S1028-4559(08)60005-4
[7]  
Chen Y, 2003, CANCER RES, V63, P1927
[8]   Identification and characterization of genes involved in the carcinogenesis of human squamous cell cervical carcinoma [J].
Cheng, Q ;
Lau, WM ;
Tay, SK ;
Chew, SH ;
Ho, TH ;
Hui, KM .
INTERNATIONAL JOURNAL OF CANCER, 2002, 98 (03) :419-426
[9]  
CHI DS, 2001, CANC MANAGEMENT MULT, P359
[10]   Gene expression profiles in squamous cell cervical carcinoma using array-based comparative genomic hybridization analysis [J].
Choi, Y. -W. ;
Bae, S. M. ;
Kim, Y. -W. ;
Lee, H. N. ;
Kim, Y. W. ;
Park, T. C. ;
Ro, D. Y. ;
Shin, J. C. ;
Shin, S. J. ;
Seo, J. -S. ;
Ahn, W. S. .
INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER, 2007, 17 (03) :687-696