Atomic Force Microscopy of Biopolymers on Graphite Surfaces

被引：7

作者：

Dubrovin, E. V. ^{[1
,2
]}

Klinov, D. V. ^{[2
,3
]}

机构：

[1] Moscow MV Lomonosov State Univ, Fac Phys, Moscow 119991, Russia

[2] Fed Med Biol Agcy, Fed Res & Clin Ctr Phys Chem Med, Moscow 119435, Russia

[3] Sirius Univ Sci & Technol, Soci 354340, Russia

来源：

POLYMER SCIENCE SERIES A | 2021年 / 63卷 / 06期

基金：

俄罗斯基础研究基金会;

关键词：

MOLECULAR-DYNAMICS SIMULATION; ORIENTED PYROLYTIC-GRAPHITE; SCANNING PROBE MICROSCOPY; SINGLE-STRANDED-DNA; HUMAN SERUM-ALBUMIN; ALPHA-C REGIONS; PROTEIN ADSORPTION; CONFORMATIONAL-CHANGES; GRAPHENE OXIDE; REAL-TIME;

D O I：

10.1134/S0965545X2106002X

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Themain peculiarities of the interaction of DNA and protein molecules with graphite are considered. The results of atomic force microscopy study of the adsorption of DNA and proteins on the surface of highly oriented pyrolytic graphite are analyzed. These data are compared with the results of molecular dynamics simulation and optical studies. The dehybridization of DNA and the denaturation of many proteins on the graphite surface which are predominantly associated with pi-pi-stacking interaction (for DNA and proteins) and van der Waals and hydrophobic interactions (for proteins) are discussed. It is shown that the surface of highly oriented pyrolytic graphite is strongly liable to random environmental contaminations that considerably change the properties of the surface and, therefore, influence the kinetics of adsorption of biopolymer molecules and their conformation on the surface. Considerable attention is given to analysis of the interaction of biopolymers with the surface of highly oriented pyrolytic graphite modified with monolayers of organic molecules.

引用

页码：601 / 622

页数：22

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