Biomimetic small exosome with outstanding surgical applications for rapid large-scale wound healing and functional sweat gland restoration

被引：10

作者：

Chen, Huating ^{[1
,2
,3
,4
,5
,6
]}

Liu, Yawei ^{[7
]}

Liu, Yiqiong ^{[1
,2
,3
,4
,5
,6
]}

Ji, Shuaifei ^{[1
,2
,3
,4
,5
,6
]}

Xiang, Jiangbing ^{[1
,2
,3
,4
,5
,6
,8
]}

Li, Yan ^{[1
,2
,3
,4
,5
,6
]}

Zhou, Laixian ^{[1
,2
,3
,4
,5
,6
]}

Gao, Huanhuan ^{[1
,2
,3
,4
,5
,6
]}

Deng, Zihui ^{[9
]}

Li, Binghui ^{[10
]}

Sun, Sujing ^{[11
]}

Cui, Shaoyuan ^{[12
]}

Li, Gongchi ^{[13
]}

Sheng, Wei ^{[14
]}

Liu, Huiling ^{[14
]}

Chen, Chunying ^{[15
]}

Zhao, Yuliang ^{[15
]}

Zhang, Hongjie ^{[7
,16
]}

Liu, Kai ^{[7
,16
]}

Fu, Xiaobing ^{[1
,2
,3
,4
,5
,6
]}

Sun, Xiaoyan ^{[1
,2
,3
,4
,5
,6
]}

机构：

[1] Chinese Peoples Liberat Army Gen Hosp, Res Ctr Tissue Repair & Regenerat, Med Innovat Res Dept, 2019RU051, Beijing 100048, Peoples R China

[2] Chinese Peoples Liberat Army Gen Hosp, Med Ctr 4, 2019RU051, Beijing 100048, Peoples R China

[3] PLA Med Coll, 2019RU051, Beijing 100048, Peoples R China

[4] Chinese Acad Med Sci, PLA Key Lab Tissue Repair & Regenerat Med, 2019RU051, Beijing 100048, Peoples R China

[5] Chinese Acad Med Sci, Beijing Key Res Lab Skin Injury Repair & Regenera, 2019RU051, Beijing 100048, Peoples R China

[6] Chinese Acad Med Sci, Res Unit Trauma Care Tissue Repair & Regenerat, 2019RU051, Beijing 100048, Peoples R China

[7] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Rare Earth Resource Utilizat, Changchun 130022, Peoples R China

[8] Chongqing Univ, Coll Bioengn, Chongqing 400044, Peoples R China

[9] Chinese Peoples Liberat Army Gen Hosp, Grad Sch, Dept Basic Med, Beijing, Peoples R China

[10] Huazhong Univ Sci & Technol, Liyuan Hosp, Tongji Med Coll, Dept Wound Repair Surg, Wuhan 430077, Hubei, Peoples R China

[11] Acad Mil Med Sci, Inst Hlth Serv & Transfus Med, Dept Emerging Transfus Technol, Beijing 100850, Peoples R China

[12] Peoples Liberat Army Gen Hosp, Med Ctr 1, Dept Nephrol, State Key Lab Kidney Dis, Beijing 100084, Peoples R China

[13] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Hand Surg, Wuhan 430022, Hubei, Peoples R China

[14] Peoples Liberat Army Gen Hosp, Med Ctr 1, Dept Tissue Regenerat & Trauma Repair, Beijing 100853, Peoples R China

[15] Natl Ctr Nanosci & Technol China, Beijing 100190, Peoples R China

[16] Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China

来源：

NANO TODAY | 2022年 / 45卷

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Biomimeticmaterials; Exosome; Woundhealing; Sweatglands; Skinregeneration; STROMAL CELLS; STEM-CELLS; SKIN; EXPRESSION;

D O I：

10.1016/j.nantod.2022.101531

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The realization of coordinated infiltration of dermal and epidermal cell types, and the rapid deposition of extracellular matrix are the prerequisites for improved healing quality of large skin defects. However, presently used materials are limited for wound healing due to the inherent poor histocompatibility, low permeability, poor mechanical property and cytotoxicity. It is challenging to develop new therapeutic agents that enable timely and efficient wound healing and meanwhile there are minimal side effects to avoid chronic wounds, wound adhesions, and large scarring. Herein, we fabricated biomimetic exosomes (EMs) that enriched transforming growth factor beta 1 (TGF-beta 1) for the rapid healing of large-scale cutaneous wounds. The EM encapsulation allowed well-managed dosing of the TGF-beta 1 to effectively promote the wound healing process by endowing epidermal keratinocytes with a migratory feature, enhancing their stem cell properties, and resulting in accelerated in vivo wound re-epithelization. Specifically, the EMs could fast restore the function of sweat glands in a thermally injured mice model. Our work provides a promising strategy for improving the healing speed and healing quality for patients with large wounds. (c) 2022 Published by Elsevier Ltd.

引用

页数：9

共 47 条

[1] Regenerative and protective effects of dMSC-sEVs on high-glucose-induced senescent fibroblasts by suppressing RAGE pathway and activating Smad pathway [J].