A loop-mediated isothermal amplification assay for the detection of Dahlia mosaic caulimovirus in Dahlia (Dahlia variabilis)

被引：9
作者：
Iftikhar, Romana [1 ]
Moyo, Lindani [1 ]
Pappu, Hanu R. [1 ]
机构：
[1] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA
来源：
ANNALS OF APPLIED BIOLOGY | 2020年 / 176卷 / 02期
关键词：
Caulimoviridae; Caulimovirus; Dahlia mosaic virus; double-stranded DNA virus; isothermal amplification; LAMP detection; PINOT-GRIS-VIRUS; DEEP SEQUENCING ANALYSIS; MOLECULAR CHARACTERIZATION; GENOME SEGMENTS; READ ALIGNMENT; PLANT-VIRUS; 1ST REPORT; GRAPEVINE; HYPOVIRULENCE; MYCOVIRUSES;
D O I：
10.1111/aab.12574
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Dahlia mosaic virus (DMV), an aphid-transmitted caulimovirus in the family Caulimoviridae, is an important pathogen of dahlia (Dahlia variabilis). Development of rapid, and sensitive diagnostic tools is essential in surveillance and management of DMV. A loop-mediated isothermal amplification (LAMP) assay was developed for DMV detection. The LAMP assay targets the reverse transcriptase region of the DMV genome and is optimal at a temperature of 60 degrees C and run time of 60 min, and amplification was detected through fluorescence detection and by agarose gel electrophoresis. The assay detected DMV in DNA concentration as low as 10(-3) ng. To the best of our knowledge, this is the first report of a LAMP assay for the detection of DMV, a plant DNA virus. This assay will be useful in rapid and sensitive detection of DMV and in reducing the virus incidence through production of virus-free planting material.
引用
收藏
页码：203 / 209
页数：7
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